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Clinical Oral Investigations
Published in: Clinical Oral Investigations 8/2020

01-08-2020 | Diode Laser | Original Article

Ex vivo histological analysis of the thermal effects created by a 445-nm diode laser in oral soft tissue biopsy

Authors: Gaspare Palaia, Alessandra Impellizzeri, Gianluca Tenore, Federico Caporali, Paolo Visca, Alessandro Del Vecchio, Gabriella Galluccio, Antonella Polimeni, Umberto Romeo

Published in: Clinical Oral Investigations | Issue 8/2020

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Abstract

Objective

The aim of this study was to evaluate, ex vivo, the histological effects of 445-nm diode laser (Eltech K-Laser srl, Treviso, Italy), during an oral soft tissue biopsy.

Materials and methods

Thirty samples from pig cadaver tongues were obtained, through five laser settings, in continuous and pulsed wave (CW and PW). Samples were divided into six groups of five pieces each. A control specimen was taken by a scalpel. All samples were examined with an optical microscope by a blinded pathologist. Thermal effects on epithelium and connective tissues were measured with LAS 4.8 software. Finally, a statistical evaluation was made using GraphPadPrism 7.0 software.

Results

All specimens, except one, showed a damage lower than 1 mm. Readability was always optimal; there was a different thermal effect between epithelial and connective tissue and in CW and PW samples, confirmed by statistical analysis too.

Conclusions

A 445-nm diode laser creates a minimum thermal effect, that has no implications in the histological evaluation of benign lesions. In suspicious lesions, a safety margin of 1 mm, compared with a scalpel, is preferable.

Clinical relevance

A 445-nm diode laser has excellent surgical properties and can manage many arduous clinical cases, such as vascularized lesions. In the excision of suspected lesions, it is necessary to compare, case by case, clinical advantages to possible histological implications.
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Metadata
Title
Ex vivo histological analysis of the thermal effects created by a 445-nm diode laser in oral soft tissue biopsy
Authors
Gaspare Palaia
Alessandra Impellizzeri
Gianluca Tenore
Federico Caporali
Paolo Visca
Alessandro Del Vecchio
Gabriella Galluccio
Antonella Polimeni
Umberto Romeo
Publication date
01-08-2020
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 8/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-019-03123-4

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