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Clinical Oral Investigations

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Open Access 01-02-2020 | Original Article

Contraction dynamics of dental pulp cell rod microtissues

Authors: Gunpreet Oberoi, Klara Janjić, Anna Sonja Müller, Barbara Schädl, Andreas Moritz, Hermann Agis

Published in: Clinical Oral Investigations | Issue 2/2020

Abstract

Objectives

The factors that contribute to the morphological changes of dental pulp cell–derived microtissues are unknown. Here, we investigated the contraction dynamics of rod-shaped microtissues derived from dental pulp cells and examined the underlying cell signaling pathways.

Methods

Human dental pulp cells were seeded into agarose molds to assemble into rod-shaped microtissues. Resazurin- and tetrazolium-based cytotoxicity assays, Live/Dead staining, and hematoxylin and eosin staining for histological evaluation of rods were performed. Rod contraction was evaluated and measured for a period of 10 days. The role of TGF-β, phosphoinositide 3-kinase (PI3K)/AKT, and mitogen-activated protein kinase (MAPK) signaling pathway was analyzed.

Results

Dental pulp cells readily assembled into rods, maintaining the geometric shape for 48 h. Following this period, they condensed to form stable spheroidal structures that remained vital for 10 days from seeding. Inhibition of phosphoinositide 3-kinase signaling pathway by LY294002 significantly prolonged the diminution in the length of rods formed by dental pulp cells. TGF-β and pharmacological inhibition of TGF-β signaling did not show pronounced effects.

Conclusion

Overall, dental pulp cells readily formed rod-shaped patterns of microtissues which, over a period of time, condensed into more stable spheroidal structures. Hence, technologies like bioprinting, using direct fabrication of microtissues need to consider the contraction dynamics.

Clinical relevance

The field of regenerative endodontology will benefit from our findings as it can be applied as a novel platform to test the impact of pharmacological agents, biomaterials, and regenerative approaches including bioprinting.

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Title: Contraction dynamics of dental pulp cell rod microtissues
Authors: Gunpreet Oberoi
Klara Janjić
Anna Sonja Müller
Barbara Schädl
Andreas Moritz
Hermann Agis
Publication date: 01-02-2020
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02917-w

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Contraction dynamics of dental pulp cell rod microtissues

Abstract

Objectives

Methods

Results

Conclusion

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusion

Clinical relevance

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