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Clinical Oral Investigations
Published in: Clinical Oral Investigations 10/2019

01-10-2019 | Cytokines | Original Article

Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment

Authors: Priyadarshni Bindal, Nareshwaran Gnanasegaran, Umesh Bindal, Nazmul Haque, Thamil Selvee Ramasamy, Wen Lin Chai, Noor Hayaty Abu Kasim

Published in: Clinical Oral Investigations | Issue 10/2019

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Abstract

Objective

In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-rich plasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.

Materials and methods

Lipopolysaccharide (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated with different concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay. Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array. Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.

Results

Viability of iDPSCs was significantly (p < 0.05) higher in 20% HPL-supplemented media compared to iDPSCs. Expression of 10 out of 12 selected angiogenic genes, four out of seven adhesion molecules, and seven out of nine cytokine-producing genes were significantly (p < 0.05) higher in cells maintained in 20% HPL-supplemented media compared to that in FBS-supplemented media. Furthermore, expression of all the selected growth factors was significantly higher (p < 0.05) in the supernatants from 20% HPL media at 12 and 24 h post-incubation.

Conclusion

This study suggests that 20% HPL could be optimum to stimulate angiogenesis-related factors in iDPSCs while maintaining their viability.

Clinical relevance

This data may suggest the potential use of 20% HPL for expanding DPSCs scheduled for clinical trials for regenerative therapies including dental pulp regeneration.
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Metadata
Title
Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment
Authors
Priyadarshni Bindal
Nareshwaran Gnanasegaran
Umesh Bindal
Nazmul Haque
Thamil Selvee Ramasamy
Wen Lin Chai
Noor Hayaty Abu Kasim
Publication date
01-10-2019
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 10/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-019-02811-5

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