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Clinical Oral Investigations
Published in: Clinical Oral Investigations 4/2019

01-04-2019 | Original Article

Effect of cold plasma on periodontal wound healing—an in vitro study

Authors: Benedikt Kleineidam, M. Nokhbehsaim, J. Deschner, G. Wahl

Published in: Clinical Oral Investigations | Issue 4/2019

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Abstract

Objectives

Cold atmospheric plasma (CAP), a room temperate ionized gas, seems to be a possible way to enhance tissue recovery. An in vitro study was conducted to investigate the influence of medical CAP on the regenerative capacity of human periodontal ligament (PDL) cells.

Material and methods

Human PDL cells were subjected to CAP at various intensities, distances, and durations. The effects of CAP on a number of specific markers were studied at transcriptional level using real-time PCR. Additionally, an in vitro wound healing assay was applied to PDL cell monolayers either in the presence or absence of CAP by using JuLI™ Br Live Cell Analyzer and software. Finally, cell viability of CAP-treated cells was analyzed by an XTT assay.

Results

CAP treatment enhanced significantly the expression of the cytokines tumor necrosis factor (TNF)α, cyclooxygenase (COX)2, interleukin (IL)-1β, IL-6, IL-8, collagen (COL)1α, and matrix metalloproteinase (MMP)1, as well as the proliferation markers Ki67 and proliferating cell nuclear antigen (PCNA), but downregulated apoptotic markers Apaf1 and p53. Additionally, the in vitro wound healing rate was significantly enhanced after CAP application. Moreover, CAP treatment resulted in a significantly increased cell viability in the XTT assay.

Conclusion

This in vitro study shows that CAP has regulatable effects on markers of periodontal wound healing thereby underlining the potential use of CAP as a benefit treatment strategy.

Clinical relevance

Our study demonstrates the application of CAP in the treatment of oral pathologies suggesting a promising future treatment approach.
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Metadata
Title
Effect of cold plasma on periodontal wound healing—an in vitro study
Authors
Benedikt Kleineidam
M. Nokhbehsaim
J. Deschner
G. Wahl
Publication date
01-04-2019
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 4/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-018-2643-3

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