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Clinical Oral Investigations

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01-04-2018 | Original Article

Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion

Authors: Jadesada Palasuk, L. Jack Windsor, Jeffrey A. Platt, Yuri Lvov, Saulo Geraldeli, Marco C. Bottino

Published in: Clinical Oral Investigations | Issue 3/2018

Abstract

Objectives

This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration.

Materials and methods

Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated.

Results

Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity.

Conclusions

The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity.

Clinical significance

Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.

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Title: Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion
Authors: Jadesada Palasuk
L. Jack Windsor
Jeffrey A. Platt
Yuri Lvov
Saulo Geraldeli
Marco C. Bottino
Publication date: 01-04-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 3/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2215-y

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical significance

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical significance

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