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01-03-2018 | Original Article

Evaluation of cortical mandibular bone in patients with oral squamous cell carcinoma

Authors: Gustavo Davi Rabelo, Claudia Coutinho-Camillo, Luiz Paulo Kowalski, Nathalie Portero-Muzy, Jean-Paul Roux, Pascale Chavassieux, Fabio Abreu Alves

Published in: Clinical Oral Investigations | Issue 2/2018

Abstract

Objectives

The aim of the study was to evaluate the mandible cortical bone changes in patients with oral squamous cell carcinoma (OSCC).

Patients and methods

Twenty patients who underwent some mandibular bone removal as part of the treatment of OSCC had bone samples collected in two parts: in the proximity of the tumor (BPT) and in the surgical margin (BEP). Cortical microarchitecture was analyzed trough micro-computed tomography, together with texture analysis, followed by microcrack evaluation in histological sections and gene expression of RANK, RANKL, OPG, and sclerostin by quantitative polymerase chain reaction.

Results

Bone surface was higher in BPT (0.005 ± 0.002 vs 0.004 ± 0.002, p = 0.01) compared with BEP. In BPT, the subset of patients without bone invasion presented higher anisotropy (0.83 ± 0.07) compared with the ones with bone invasion (0.70 ± 0.14) (p = 0.04). RANK, RANKL, OPG, and sclerostin were found to be downregulated in the majority of cases in both parts. There were significant correlations between the parameters of microarchitecture and gene expression analysis (p < 0.001 to p < 0.05), most of them related with OPG levels.

Conclusion

The cortex in the mandible in the proximity of the tumor reveals more bone surface than the bone in the surgical margin, and the tumor invasion causes a decrease in anisotropy. RANK, RANKL, OPG, and sclerostin are downregulated in mandible, in both parts analyzed. Correlation tests revealed the association between cortical thickness, bone surface, anisotropy, porosity, bone mineral density, and OPG levels.

Clinical relevance

The mandible cortical bone microarchitecture changes in the proximity of the squamous cell carcinoma lesion.

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Title: Evaluation of cortical mandibular bone in patients with oral squamous cell carcinoma
Authors: Gustavo Davi Rabelo
Claudia Coutinho-Camillo
Luiz Paulo Kowalski
Nathalie Portero-Muzy
Jean-Paul Roux
Pascale Chavassieux
Fabio Abreu Alves
Publication date: 01-03-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2153-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Evaluation of cortical mandibular bone in patients with oral squamous cell carcinoma

Abstract

Objectives

Patients and methods

Results

Conclusion

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Patients and methods

Results

Conclusion

Clinical relevance

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