Skip to main content
Top
Published in: Clinical Oral Investigations 6/2015

01-07-2015 | Original Article

Endodontic retreatment: clinical comparison of reciprocating systems versus rotary system in disinfecting root canals

Authors: Frederico C. Martinho, Lilian F. Freitas, Gustavo G. Nascimento, Aleteia M Fernandes, Fabio R. M. Leite, Ana P. M. Gomes, Izabel C G Camões

Published in: Clinical Oral Investigations | Issue 6/2015

Login to get access

Abstract

Introduction

This clinical study was conducted to compare the effectiveness of single-file reciprocating systems and rotary systems in removing endotoxins and cultivable bacteria in endodontic retreatment.

Methods

Thirty endodontically treated teeth with post-treatment apical periodontitis were selected. The specimens were divided into three groups according to the system used: WaveOne (n = 10), Reciproc instrument (n = 10), and ProTaper Universal Retreatment system (n = 10). Samples were collected before and after chemomechanical preparation. The irrigation was performed by using 2.5 % sodium hypochlorite. A chromogenic limulus amebocyte lysate assay test was used to quantify endotoxins. Culture techniques were used to determine bacterial colony-forming unit counts.

Results

At baseline, endotoxins and cultivable bacteria were recovered from 100 % of the root canal samples in a median value of 5.84 EU/mL and 4.98 × 103 CFU/mL, respectively. After CMP, no differences were found in the median percentage values of endotoxin reduction achieved with reciprocating systems—WaveOne [94.11 %] and Reciproc [93.29 %] and with rotary systems—ProTaper [94.98 %] (P > 0.05). Both single-file reciprocating systems [WaveOne (98.27 %) and Reciproc (99.54 %)] and rotary system [ProTaper (98.73 %)] were effective in reducing bacterial load (P > 0.05). Moreover, no differences were found among the systems tested.

Conclusions

The Reciproc and WaveOne reciprocating systems were as effective as the ProTaper system for removal of endotoxins and bacteria in endodontic retreatment.

Clinical relevance

All systems tested were effective to remove cultivable bacteria and endotoxin in endodontic retreatment. As no differences among systems were observed, it is possible to suggest that clinicians should choose the preferred technique to perform endodontic.
Literature
1.
go back to reference Siqueira JF Jr, Lopes HP (2011) Chemomechanical preparation. In: Siqueira JF Jr (ed) Treatment of endodontic infections. Quintessence Publishing, London Siqueira JF Jr, Lopes HP (2011) Chemomechanical preparation. In: Siqueira JF Jr (ed) Treatment of endodontic infections. Quintessence Publishing, London
2.
go back to reference Siqueira JF Jr, Alves FR, Versiani MA, Rôças IN, Almeida BM, Neves MA, Sousa-Neto MD (2013) Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod 39:1044–1050. doi:10.1016/j.joen.2013.04.034 PubMedCrossRef Siqueira JF Jr, Alves FR, Versiani MA, Rôças IN, Almeida BM, Neves MA, Sousa-Neto MD (2013) Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod 39:1044–1050. doi:10.​1016/​j.​joen.​2013.​04.​034 PubMedCrossRef
3.
go back to reference Barbizam JV, Fariniuk LF, Marchesan MA, Pecora JD, Sousa-Neto MD (2002) Effectiveness of manual and rotary instrumentation techniques for cleaning flattened root canals. J Endod 28:365–366PubMedCrossRef Barbizam JV, Fariniuk LF, Marchesan MA, Pecora JD, Sousa-Neto MD (2002) Effectiveness of manual and rotary instrumentation techniques for cleaning flattened root canals. J Endod 28:365–366PubMedCrossRef
4.
5.
go back to reference Peters OA, Schönenberger K, Laib A (2001) Effects of four Ni-Ti preparation techniques on root canal geometry assessed by micro computed tomography. Int Endod J 34:221–230PubMedCrossRef Peters OA, Schönenberger K, Laib A (2001) Effects of four Ni-Ti preparation techniques on root canal geometry assessed by micro computed tomography. Int Endod J 34:221–230PubMedCrossRef
7.
go back to reference Endo MS, Martinho FC, Zaia AA, Ferraz CC, Almeida JF, Gomes BP (2012) Quantification of cultivable bacteria and endotoxin in post-treatment apical periodontitis before and after chemo-mechanical preparation. Eur J Clin Microbiol Infect Dis 31:2575–2583. doi:10.1007/s10096-012-1598-6 PubMedCrossRef Endo MS, Martinho FC, Zaia AA, Ferraz CC, Almeida JF, Gomes BP (2012) Quantification of cultivable bacteria and endotoxin in post-treatment apical periodontitis before and after chemo-mechanical preparation. Eur J Clin Microbiol Infect Dis 31:2575–2583. doi:10.​1007/​s10096-012-1598-6 PubMedCrossRef
8.
go back to reference Bramante CM, Fidelis NS, Assumpção TS, Bernardineli N, Garcia RB, Bramante AS, de Moraes IG (2010) Heat release, time required, and cleaning ability of MTwo R and ProTaper universal retreatment systems in the removal of filling material. J Endod 36:1870–1873. doi:10.1016/j.joen.2010.08.013 PubMedCrossRef Bramante CM, Fidelis NS, Assumpção TS, Bernardineli N, Garcia RB, Bramante AS, de Moraes IG (2010) Heat release, time required, and cleaning ability of MTwo R and ProTaper universal retreatment systems in the removal of filling material. J Endod 36:1870–1873. doi:10.​1016/​j.​joen.​2010.​08.​013 PubMedCrossRef
10.
go back to reference Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, Haapasalo M (2012) Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod 38:1516–1520. doi:10.1016/j.joen.2012.08.001 PubMedCrossRef Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, Haapasalo M (2012) Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod 38:1516–1520. doi:10.​1016/​j.​joen.​2012.​08.​001 PubMedCrossRef
11.
go back to reference Machado ME, Nabeshima CK, Leonardo MF, Reis FA, Britto ML, Cai S (2013) Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J 46:1083–1087. doi:10.1111/iej.12108 PubMed Machado ME, Nabeshima CK, Leonardo MF, Reis FA, Britto ML, Cai S (2013) Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J 46:1083–1087. doi:10.​1111/​iej.​12108 PubMed
16.
go back to reference Fruchi Lde C, Ordinola-Zapata R, Cavenago BC, Hungaro Duarte MA, da Silveira Bueno CE, De Martin AS (2014) Efficacy of reciprocating instruments for removing filling material in curved canals obturated with a single-cone technique: a micro-computed tomographic analysis. J Endod 40:1000–1004. doi:10.1016/j.joen.2013.12.011 PubMedCrossRef Fruchi Lde C, Ordinola-Zapata R, Cavenago BC, Hungaro Duarte MA, da Silveira Bueno CE, De Martin AS (2014) Efficacy of reciprocating instruments for removing filling material in curved canals obturated with a single-cone technique: a micro-computed tomographic analysis. J Endod 40:1000–1004. doi:10.​1016/​j.​joen.​2013.​12.​011 PubMedCrossRef
18.
go back to reference Martinho FC, Gomes AP, Fernandes AM, Ferreira NS, Endo MS, Freitas LF, Camões IC (2014) Clinical comparison of the effectiveness of single-file reciprocating systems and rotary systems for removal of endotoxins and cultivable bacteria from primarily infected root canals. J Endod 40:625–629. doi:10.1016/j.joen.2013.12.006 PubMedCrossRef Martinho FC, Gomes AP, Fernandes AM, Ferreira NS, Endo MS, Freitas LF, Camões IC (2014) Clinical comparison of the effectiveness of single-file reciprocating systems and rotary systems for removal of endotoxins and cultivable bacteria from primarily infected root canals. J Endod 40:625–629. doi:10.​1016/​j.​joen.​2013.​12.​006 PubMedCrossRef
19.
go back to reference Buchanan LS (2000) The standardized-taper root canal preparation–Part 1. Concepts for variably tapered shaping instruments. Int Endod J 33:516–529PubMedCrossRef Buchanan LS (2000) The standardized-taper root canal preparation–Part 1. Concepts for variably tapered shaping instruments. Int Endod J 33:516–529PubMedCrossRef
22.
go back to reference Martinho FC, Chiesa WM, Marinho AC, Zaia AA, Ferraz CC, Almeida JF, Souza-Filho FJ, Gomes BP (2010) Clinical investigation of the efficacy of chemomechanical preparation with rotary nickel-titanium files for removal of endotoxin from primarily infected root canals. J Endod 36:1766–1769. doi:10.1016/j.joen.2010.08.019 PubMedCrossRef Martinho FC, Chiesa WM, Marinho AC, Zaia AA, Ferraz CC, Almeida JF, Souza-Filho FJ, Gomes BP (2010) Clinical investigation of the efficacy of chemomechanical preparation with rotary nickel-titanium files for removal of endotoxin from primarily infected root canals. J Endod 36:1766–1769. doi:10.​1016/​j.​joen.​2010.​08.​019 PubMedCrossRef
23.
go back to reference Möller AJR (1966) Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr 74:1–380PubMed Möller AJR (1966) Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr 74:1–380PubMed
24.
25.
go back to reference Nair PN, Sjögren U, Figdor D, Sundqvist G (1999) Persistent periapical radiolucencies of root-filled human teeth, failed endodontic treatments, and periapical scars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 87:617–627PubMedCrossRef Nair PN, Sjögren U, Figdor D, Sundqvist G (1999) Persistent periapical radiolucencies of root-filled human teeth, failed endodontic treatments, and periapical scars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 87:617–627PubMedCrossRef
26.
go back to reference Cheung GS, Ho MW (2001) Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol 16:332–337PubMedCrossRef Cheung GS, Ho MW (2001) Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol 16:332–337PubMedCrossRef
27.
go back to reference Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M (2001) Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 34:429–434PubMedCrossRef Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M (2001) Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 34:429–434PubMedCrossRef
31.
34.
35.
go back to reference Marinho AC, Martinho FC, Zaia AA, Ferraz CC, Gomes BP (2012) Influence of the apical enlargement size on the endotoxin level reduction of dental root canals. J Appl Oral Sci 20:661–666PubMedCentralPubMedCrossRef Marinho AC, Martinho FC, Zaia AA, Ferraz CC, Gomes BP (2012) Influence of the apical enlargement size on the endotoxin level reduction of dental root canals. J Appl Oral Sci 20:661–666PubMedCentralPubMedCrossRef
Metadata
Title
Endodontic retreatment: clinical comparison of reciprocating systems versus rotary system in disinfecting root canals
Authors
Frederico C. Martinho
Lilian F. Freitas
Gustavo G. Nascimento
Aleteia M Fernandes
Fabio R. M. Leite
Ana P. M. Gomes
Izabel C G Camões
Publication date
01-07-2015
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 6/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-014-1360-9

Other articles of this Issue 6/2015

Clinical Oral Investigations 6/2015 Go to the issue