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Clinical Oral Investigations
Published in: Clinical Oral Investigations 2/2014

01-03-2014 | Original Article

Immunohistochemical analysis of staged guided bone regeneration and osseointegration of titanium implants using a polyethylene glycol membrane

Authors: Ilja Mihatovic, Vladimir Golubovic, Jürgen Becker, Frank Schwarz

Published in: Clinical Oral Investigations | Issue 2/2014

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Abstract

Objectives

This study aimed to immunohistochemically evaluate staged guided bone regeneration and osseointegration of titanium implants using two bone graft substitutes in combination with a polyethylene glycol (PEG) membrane in a dog model.

Materials and methods

Saddle-type alveolar ridge defects were prepared in the lower jaws of 12 foxhounds and randomly filled with a natural bone mineral (NBM) or a biphasic calcium phosphate (SBC) and covered with an in situ gelling PEG membrane. After a healing period of 8 and 12 weeks (six animals each), modSLA titanium implants were inserted to heal in a submerged position. At 8 + 2 and 12 + 2 weeks, respectively, dissected blocks were processed for immunohistochemical analysis [osteocalcin (OC)].

Results

After 8 + 2 weeks, mean OC values (%) tended to be higher in the NBM group (NBM, 32.7 ± 8.9 %), but failed to reach statistical significance over the SBC group (SBC, 24.4 ± 6.6 %). After 12 + 2 weeks, mean OC values decreased in both groups and was almost identical in both groups (NBM 1.6 ± 1.2 %/SBC 2.1 ± 1.4 %).

Conclusion

It was concluded that all augmentation procedures investigated were characterised by a comparable OC activity during the process of bone regeneration and osseointegration of modSLA titanium implants.
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Metadata
Title
Immunohistochemical analysis of staged guided bone regeneration and osseointegration of titanium implants using a polyethylene glycol membrane
Authors
Ilja Mihatovic
Vladimir Golubovic
Jürgen Becker
Frank Schwarz
Publication date
01-03-2014
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 2/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-013-0995-2

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