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Clinical Oral Investigations

Published in:

01-01-2014 | Original Article

Biomimetic treatment on dental implants for short-term bone regeneration

Authors: Francisco Javier Gil, Norberto Manzanares, Armando Badet, Conrado Aparicio, Maria-Pau Ginebra

Published in: Clinical Oral Investigations | Issue 1/2014

Abstract

Objectives

The main purpose of this work was to assess the short-term bone regenerative potential of new osteoconductive implants. The novelty of the study lies in the analysis of the effectiveness of a novel two-step treatment which combines shot-blasting with a thermo-chemical treatment, at very short times after implant placement in a minipig model.

Materials and methods

Three hundred twenty implants with four different surface treatments, namely bioactivated surfaces, micro-rough grit-blasted, micro-rough acid-etched and smooth as-machined titanium implants were placed into the bone of 20 minipigs. The percent of bone-to-implant contact was determined 3 days, 1, 2, 3 and 10 weeks after implant placement by histomorphometric analysis. Surface composition, topography and wettability of the implant specimens were analysed.

Results

The combination of shot-blasting and thermo-chemical treatment accelerated bone regeneration at early stages in comparison with all other treatments between day 3 and week 3 (p < 0.05). The value of osseointegration attained at week 2 was maintained until the end of the experiment without any significant changes (percent direct contact ≈ 85 %). This was mostly attributed to the ability of these implants to form in vivo a layer of apatitic mineral that coated the implant and could rapidly stimulate bone nucleation and growth from the implant surface.

Conclusions

The surface quality resulting from this treatment on cpTi provided dental implants with a unique ability of rapid bone regeneration and osseointegration.

Clinical relevance

This treatment represents a step forward in the direction of reducing the time prior to implant loading.

Wang HL, Ormianer Z, Palti A (2006) Consensus conference on immediate loading: the single tooth and partial edentulous areas. Impl Dent 15:324–333CrossRef

Ericsson I, Nilson H, Nilner K (2001) Immediate functional loading of Branemarksingle-tooth implants. A 5-year clinical follow-up. Appl Osseoint Res 2:12–17

Assad A, Hassan S, Shawky Y (2007) Clinical and radiographic evaluation of implant-retained mandibular overdentures with immediate loading. Impl Dent 16:212–218CrossRef

Gapski R, Wang HL, Mascarenhas P (2003) Critical review of immediate implant loading. Clin Oral Impl Res 14:515–527CrossRef

Stavropoulos A, Nyengaard JR, Lang NP, Karring T (2006) Immediate loading of single SLA implants: osteotomes vs. drilling. Clin Oral Impl Res 17:35–36CrossRef

Mcglumphy E, Philips K, Schliephake H, Wang IC, Chacon G, Larsen P (2006) Implant stability in the posterior mandible following an early protocol using the microthread osseospeed fixture. Clin Oral Impl Res 17:102–103CrossRef

Mangano F, Mangano C, Piatelli A, Iezzi G, Perrotti V (2006) Histologic evaluation of immediately loaded titanium implants. Clin Oral Impl Res 17:52–53CrossRef

Bernard JP, Belser UC, Martinet JP (1995) Osseointegration of Branemark fixtures using a single-step operating technique. A preliminary prospective one-year study in the edentulous mandible. Clin Oral Impl Res 6:122–129CrossRef

Chiapasco M, Gatti C (2004) Immediate loading of dental implants placed in revascularized fibula free flaps: a clinical report on 2 consecutive patients. Int J Oral Maxillofac Implants 19:906–912PubMed

10.

Chaushu G, Chaushu S, Tzohar A (2001) Immediate loading of singletooth implants: immediate versus non-immediate implantation: a clinical report. Int J Oral Maxillofac Implants 16:267–272PubMed

11.

Spiekermann H, Jansen VK, Richter EJ (1995) A 10-year follow-up study of IMZ and TPS implants in the edentulous mandible using bar retained overdentures. Int J Oral Maxillofac Implants 10:231–243PubMed

12.

Romanos GE (2003) Treatment of advanced periodontal destruction with immediately loaded implants and simultaneous bone augmentation: a case report. J Periodontol 74:255–261PubMedCrossRef

13.

McCarthy C, Patel RR, Wragg PF (2003) Sinus augmentation bone grafts for the provision of dental implants: report of clinical outcome. Int J Oral Maxillofac Implants 18:377–382PubMed

14.

Degidi M, Piatelli A (2005) 7-year follow-up of 93 immediately loaded titanium dental implants. J Oral Impl 31:25–31CrossRef

15.

Chiapasco M (2004) Early and immediate restoration and loading of implants in completely edentulous patients. Int J Oral Maxillofac Implants 19(Suppl):76–91PubMed

16.

Fischer K, Stenberg T (2004) Early loading of ITI implants supporting maxillary full-arch prosthesis: 1-year data of a prospective, randomized study. Int J Oral Maxillofac Implants 19:374–381PubMed

17.

Jokstad A, Braeffer U, Brunski JB (2003) Quality of dental implants. Int Dent J 53:409–443PubMed

18.

Calandriello R, Tomatis M, Vallone R (2003) Immediate occlusal loading of single lower molars using Branemark System Wide-Platform TiUnite implants: an interim report of a prospective open-ended clinical multicenter study. Clin Impl Dent Rel Res 5:74–80CrossRef

19.

Cornelini R, Cangini F, Covani U (2004) Immediate restoration of single tooth implants in mandibular molar sites: a 12-month preliminary report. Int J Oral Maxillofac Implants 19:855–860PubMed

20.

Roynesdal AK, Amundrud B, Haanaes HR (2001) A comparative clinical investigation of 2 early loaded ITI dental implants supporting an overdenture in the mandible. Int J Oral Maxillofac Implants 16:246–251PubMed

21.

Rocci A, Martignoni M, Gottlow J (2003) Immediate loading in the maxilla using flapless surgery, implants placed in predetermined positions, and prefabricated provisional restorations: a retrospective 3-year clinical study. Clin Impl Dent Rel Res 5:29–36CrossRef

22.

Packer ME, Watson RM, Bryant CJ (2000) A comparison of the early postoperative care required by patients treated with single and two-stage surgical techniques for the provision of Branemark implant-supported mandibular overdentures. Eur J Prosth Rest Dent 8:17–21

23.

Kupeyan HK, Shaffner M, Armstrong J (2006) Definitive CAD/CAM-guided prosthesis for immediate loading of bone-grafted maxilla: a case report. Clin Impl Dent Rel Res 8:161–167CrossRef

24.

Glauser R, Ree A, Lundgren A (2001) Immediate occlusal loading of Branemark implants applied in various jawbone regions: a prospective, 1-year clinical study. Clin Impl Dent Rel Res 3:204–213CrossRef

25.

Pegueroles M, Aguirre A, Engel E, Pavon G, Gil FJ, Planell JA, Migonney V, Aparicio C (2001) Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: a gene expression study using real-time RT-PCR. J Mater Sci Mater Med 22:617–627CrossRef

26.

Pegueroles M, Aparicio C, Bosio M, Engel E, Gil FJ, Planell JA, Altankov G (2010) Spatial organization of osteoblast fibronectin-matrix on titanium surface—effects of roughness, chemical heterogeneity, and surface free energy. Acta Biomater 6:291–301PubMedCrossRef

27.

Boyan BD, Schwartz Z (1999) Modulation of osteogenesis via implant surface design. In: Davies JE (ed) Bone engineering. Em squared Inc., Toronto, Canada, pp 232–239

28.

Buser D (1991) Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs. J Biomed Mater Res 25:889–902PubMedCrossRef

29.

Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T (1990) Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic. J Biomed Mater Res 24:721–728PubMedCrossRef

30.

Aparicio C, Gil FJ, Planell JA, Engel E (2002) Human ostoeblast proliferation and differentiation on grit-blasted and bioactive titanium for dental applications. J Mater Sci Mater Med 13:1105–1111PubMedCrossRef

31.

Aparicio C, Gil FJ, Fonseca C, Barbosa M, Planell JA (2003) Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot blasted with different materials and sizes of shot particles for dental implant applications. Biomaterials 24:263–273PubMedCrossRef

32.

Aparicio C, Gil FJ, Thams U, Munoz F, Padros A, Planell JA (2004) Osseointegration of grit-blasted and bioactive titanium implants: histomorphometry in minipigs. Key Eng Mat 254–2:737–740CrossRef

33.

Aparicio C, Manero JM, Conde F, Pegueroles M, Planell JA, Vallet-Regí M, Gil FJ (2007) Acceleration of apatite nucleation on microrough bioactive titanium for bone-replacing implants. J Biomed Mater Res 82A:521–529CrossRef

34.

Kokubo T, Miyaji F, Kim HM (1996) Preparation of bioactive Ti and its alloys via simple chemical surface treatment. J Am Ceram Soc 79:1127–1129CrossRef

35.

Kokubo T (1997) Bioactive implants. Anales de Química Int Ed 93:49–55

36.

Albrektsson T, Brånemark PI, Hansson BO, Lindström J (1981) Osseointegrated dental implants. Acta Orthop Scand 52:155–170PubMedCrossRef

37.

Nogueras J, Gil FJ, Salsench J, Martínez-Gomis J (2004) Roughness and bonding strength of bioactive apatite layer on dental implants. Impl Dent 13:185–189CrossRef

38.

Thomsen P, Larsson C, Ericsson LE, Sennerby L, Lausmaa J, Kasemo B (1997) Bone response to machined cast titanium implants. J Mater Sci Mater Med 8:653–665PubMedCrossRef

39.

Yan WQ, Nakamura T, Kobayashi M, Kim HM, Miyaji F, Kokubo T (1997) Apatite-forming ability of CaO-containing titania. J Biomed Mater Res 37:265–275CrossRef

40.

Ohtsuki C, Iida H, Hayakawa S, Osaka A (1998) Bioactivity of titanium treated with hydrogen peroxide solutions containing metal chlorides. J Biomed Mater Res 39:141–152CrossRef

41.

Letankov G, Groth T (1996) Fibronectin matrix formation by human fibroblasts on surfaces varying in wettability. J Biomat Sci Pol Ed 8:299–310

42.

Groessnerschreiber B, Tuan RS (1992) Enhanced extracellular-matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro. J Cell Sci 101:209–211

43.

Brugge PJ, Torensma R, De Ruijter JE, Figdor CG, Jansen JA (2002) Modulation of integrin expression on rat bone marrow cells by substrates with different surface characteristics. Tissue Eng 8:615–626PubMedCrossRef

44.

Kieswetter K, Schwartz Z, Hummert TW, Cochran DL, Simpson J, Dean DD, Boyan BD (1996) Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater Res 32:55–63PubMedCrossRef

45.

Martin JY, Schwartz Z, Hummert TW, Schraub DM, Simpson J, Lankford J, Dean DD, Cochran DL, Boyan B (1995) Effect of titanium surface-roughness on proliferation, differentiation, and protein-synthesis of human osteoblast-like cells (Mg63). J Biomed Mater Res 29:389–401PubMedCrossRef

46.

Altankov G, Grinnell F, Groth T (1996) Studies on the biocompatibility of materials: fibroblast reorganization of substratum-bound fibronectin on surfaces varying in wettability. J Biomed Mater Res 30:385–391PubMedCrossRef

47.

Tzoneva R, Groth T, Altankov G, Paul D (2002) Remodeling of fibrinogen by endothelial cells in dependence on fibronectin matrix assembly. Effect of substratum wettability. J Mater Sci Mater Med 13:1235–1244PubMedCrossRef

48.

Altankov G, Groth T (1996) Fibronectin matrix formation and the biocompatibility of materials. J Mater Sci Mater Med 7:425–429CrossRef

49.

Altankov G, Richau K, Groth T (2003) The role of surface zeta potential and substratum chemistry for regulation of dermal fibroblasts interaction. Material Werkst 34:1120–1128CrossRef

50.

Faucheux N, Schweiss R, Lutzow K, Werner C, Groth T (2004) Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies. Biomaterials 25:2721–2730PubMedCrossRef

51.

Garcia AJ (2005) Get a grip: integrins in cell–biomaterial interactions. Biomaterials 26:7525–7529PubMedCrossRef

52.

Lebaron RG, Athanasiou KA (2000) Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials. Tissue Eng 6:85–103PubMedCrossRef

53.

Nordahl J, Mengarelliwidholm S, Hultenby K, Reinholt FP (1995) Ultrastructural immunolocalization of fibronectin in epiphyseal and metaphyseal bone of young-rats. Calcif Tissue Int 57:442–449PubMedCrossRef

54.

Vogel V, Baneyx G (2003) The tissue engineering puzzle: a molecular perspective. Ann Rev Biomed Eng 5:441–463CrossRef

55.

Globus RK, Doty SB, Lull JC, Holmuhamedov E, Humphries MJ, Damsky CH (1998) Fibronectin is a survival factor for differentiated osteoblasts. J Cell Sci 111:1385–1393PubMed

Title: Biomimetic treatment on dental implants for short-term bone regeneration
Authors: Francisco Javier Gil
Norberto Manzanares
Armando Badet
Conrado Aparicio
Maria-Pau Ginebra
Publication date: 01-01-2014
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 1/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-013-0953-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Biomimetic treatment on dental implants for short-term bone regeneration

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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