Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Journal of Bone and Mineral Metabolism
Published in: Journal of Bone and Mineral Metabolism 4/2010

01-07-2010 | Original Article

Mineral maturity and crystallinity index are distinct characteristics of bone mineral

Authors: Delphine Farlay, Gérard Panczer, Christian Rey, Pierre D. Delmas, Georges Boivin

Published in: Journal of Bone and Mineral Metabolism | Issue 4/2010

Login to get access

Abstract

The purpose of this study was to test the hypothesis that mineral maturity and crystallinity index are two different characteristics of bone mineral. To this end, Fourier transform infrared microspectroscopy (FTIRM) was used. To test our hypothesis, synthetic apatites and human bone samples were used for the validation of the two parameters using FTIRM. Iliac crest samples from seven human controls and two with skeletal fluorosis were analyzed at the bone structural unit (BSU) level by FTIRM on sections 2–4 μm thick. Mineral maturity and crystallinity index were highly correlated in synthetic apatites but poorly correlated in normal human bone. In skeletal fluorosis, crystallinity index was increased and maturity decreased, supporting the fact of separate measurement of these two parameters. Moreover, results obtained in fluorosis suggested that mineral characteristics can be modified independently of bone remodeling. In conclusion, mineral maturity and crystallinity index are two different parameters measured separately by FTIRM and offering new perspectives to assess bone mineral traits in osteoporosis.
Literature
1.
Green J (1994) The physicochemical structure of bone: cellular and noncellular elements. Miner Electrolyte Metab 20:7–15PubMed
2.
Cazalbou S (2000) Ph.D. thesis. University of Toulouse, France
3.
Cazalbou S, Combes C, Eichert D, Rey C, Glimcher MJ (2004) Poorly crystalline apatites: evolution and maturation in vitro and in vivo. J Bone Miner Metab 22:310–317CrossRefPubMed
4.
Barry AB, Baig AA, Miller SC, Higuchi WI (2002) Effect of age on rat bone solubility and crystallinity. Calcif Tissue Int 71:167–171CrossRefPubMed
5.
Ager JW, Nalla RK, Breeden KL, Ritchie RO (2005) Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone. J Biomed Opt 10:034012CrossRefPubMed
6.
Akkus O, Polyakova-Akkus A, Adar F, Schaffler MB (2003) Aging of microstructural compartments in human compact bone. J Bone Miner Res 18:1012–1019CrossRefPubMed
7.
Boskey AL, DiCarlo E, Paschalis E, West P, Mendelsohn R (2005) Comparison of mineral quality and quantity in iliac crest biopsies from high- and low-turnover osteoporosis: an FT-IR microspectroscopic investigation. Osteoporos Int 16:2031–2038CrossRefPubMed
8.
Legeros RZ (1981) Apatites in biological systems. Prog Crystal Growth Charact 4:1–45CrossRef
9.
Miller LM, Vairavamurthy V, Chance MR, Mendelsohn R, Paschalis EP, Betts F, Boskey AL (2001) In situ analysis of mineral content and crystallinity in bone using infrared micro-spectroscopy of the ν(4) PO4 3− vibration. Biochim Biophys Acta 1527:11–19PubMed
10.
Paschalis EP, DiCarlo E, Betts F, Sherman P, Mendelsohn R, Boskey AL (1996) FTIR microspectroscopic analysis of human osteonal bone. Calcif Tissue Int 59:480–487PubMed
11.
Pleshko N, Boskey A, Mendelsohn R (1991) Novel infrared spectroscopic method for the determination of crystallinity of hydroxyapatite minerals. Biophys J 60:786–793CrossRefPubMed
12.
Rey C, Shimizu M, Collins B, Glimcher MJ (1990) Resolution-enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ions in the early deposits of a solid phase of calcium-phosphate in bone and enamel, and their evolution with age. I. Investigations in the upsilon 4 PO4 domain. Calcif Tissue Int 46:384–394CrossRefPubMed
13.
Rey C, Shimizu M, Collins B, Glimcher MJ (1991) Resolution-enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ion in the early deposits of a solid phase of calcium phosphate in bone and enamel and their evolution with age. 2. Investigations in the ν3PO4 domain. Calcif Tissue Int 49:383–388CrossRefPubMed
14.
Akkus O, Adar F, Schaffler MB (2004) Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone. Bone (NY) 34:443–453
15.
Morris MD, Finney WF, Rajachar RM, Kohn DH (2004) Bone tissue ultrastructural response to elastic deformation probed by Raman spectroscopy. Faraday Discuss 126:159–168 discussion 169–183CrossRefPubMed
16.
Tarnowski CP, Ignelzi MA Jr, Wang W, Taboas JM, Goldstein SA, Morris MD (2004) Earliest mineral and matrix changes in force-induced musculoskeletal disease as revealed by Raman microspectroscopic imaging. J Bone Miner Res 19:64–71CrossRefPubMed
17.
Carden A, Rajachar RM, Morris MD, Kohn DH (2003) Ultrastructural changes accompanying the mechanical deformation of bone tissue: a Raman imaging study. Calcif Tissue Int 72:166–175CrossRefPubMed
18.
Miller LM, Little W, Schirmer A, Sheik F, Busa B, Judex S (2007) Accretion of bone quantity and quality in the developing mouse skeleton. J Bone Miner Res 22:1037–1045CrossRefPubMed
19.
Carden A, Morris MD (2000) Application of vibrational spectroscopy to the study of mineralized tissues (review). J Biomed Opt 5:259–268CrossRefPubMed
20.
Fratzl P, Gupta HS, Paschalis EP, Roschger P (2004) Structure and mechanical quality of the mineral quality of the collagen-mineral nano-composite in bone. J Mater Chem 14:2115–2123CrossRef
21.
Paschalis EP, Glass EV, Donley DW, Eriksen EF (2005) Bone mineral and collagen quality in iliac crest biopsies of patients given teriparatide: new results from the fracture prevention trial. J Clin Endocrinol Metab 90:4644–4649CrossRefPubMed
22.
Miller LM, Novatt JT, Hamerman D, Carlson CS (2004) Alterations in mineral composition observed in osteoarthritic joints of cynomolgus monkeys. Bone (NY) 35:498–506
23.
Huang RY, Miller LM, Carlson CS, Chance MR (2003) In situ chemistry of osteoporosis revealed by synchrotron infrared microspectroscopy. Bone (NY) 33:514–521
24.
Rey C, Hina A, Tofighi A, Glimcher MJ (1995) Maturation of poorly crystalline apatites: chemical and structural aspects in vivo and in vitro. Cells Mater 5:345–356
25.
Gee A, Dietz VR (1955) Pyrophosphate formation upon ignition of precipitated basic calcium phosphate. J Am Chem Soc 77:2961–2965CrossRef
26.
Shemesh A (1990) Crystallinity and diagenesis of sedimentary apatites. Geochim Cosmochim Acta 54:2433–2438CrossRef
27.
Termine JD, Posner AS (1966) Infra-red determination of the percentage of crystallinity in apatitic calcium phosphates. Nature (Lond) 211:268–270CrossRef
28.
Frazier PD, Little MF, Casciani FS (1967) X-ray diffraction analysis of human enamel containing different amounts of fluoride. Arch Oral Biol 12:35–42CrossRefPubMed
29.
Bohic S, Heymann D, Pouezat JA, Gauthier O, Daculsi G (1998) Transmission FT-IR microspectroscopy of mineral phases in calcified tissues. C R Acad Sci III 321:865–876PubMed
30.
Gadaleta SJ, Paschalis EP, Betts F, Mendelsohn R, Boskey AL (1996) Fourier transform infrared spectroscopy of the solution-mediated conversion of amorphous calcium phosphate to hydroxyapatite: new correlations between X-ray diffraction and infrared data. Calcif Tissue Int 58:9–16CrossRefPubMed
31.
Camacho NP, Rinnerthaler S, Paschalis EP, Mendelsohn R, Boskey AL, Fratzl P (1999) Complementary information on bone ultrastructure from scanning small angle X-ray scattering and Fourier-transform infrared microspectroscopy. Bone (NY) 25:287–293
32.
Paschalis EP, Betts F, DiCarlo E, Mendelsohn R, Boskey AL (1997) FTIR microspectroscopic analysis of human iliac crest biopsies from untreated osteoporotic bone. Calcif Tissue Int 61:487–492CrossRefPubMed
33.
Paschalis EP, Betts F, DiCarlo E, Mendelsohn R, Boskey AL (1997) FTIR microspectroscopic analysis of normal human cortical and trabecular bone. Calcif Tissue Int 61:480–486CrossRefPubMed
34.
Glimcher MG (1998) The nature of the mineral phase in bone: biological and clinical implications. In: Avioli LV, Krane SM (eds) Metabolic bone disease. Academic Press, San Diego, pp 23–50
35.
Jager C, Welzel T, Meyer-Zaika W, Epple M (2006) A solid-state NMR investigation of the structure of nanocrystalline hydroxyapatite. Magn Reson Chem 44:573–580CrossRefPubMed
36.
Boskey A, Maresca M, Appel J (1989) The effects of noncollagenous matrix proteins on hydroxyapatite formation and proliferation in a collagen gel system. Connect Tissue Res 21:171–176 discussion 177–178CrossRefPubMed
37.
Boskey AL (1989) Noncollagenous matrix proteins and their role in mineralization. Bone Miner 6:111–123CrossRefPubMed
38.
Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G (1998) Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone (NY) 23:187–196
39.
Dziak KL, Akkus O (2008) Effects of polyelectrolytic peptides on the quality of mineral crystals grown in vitro. J Bone Miner Metab 26:569–575CrossRefPubMed
40.
Rey C, Beshah K, Griffin R, Glimcher MJ (1991) Structural studies of the mineral phase of calcifying cartilage. J Bone Miner Res 6:515–525CrossRefPubMed
41.
Pucéat E, Reynard B, Lécuyer C (2004) Can crystallinity be used to determine the degree of chemical alteration of biogenic apatites? Chem Geol 205:83–97CrossRef
42.
Runt J, Kanchanasopa M. Crystallinity determination. In: Encyclopedia of polymer science and technology, vol 9. Wiley, New York, pp 446–464
43.
Ferrari AC, Robertson J (2004) Raman spectroscopy of amorphous nanostructured, diamond-like carbon, and nanodiamond. Philos Trans R Soc Lond A 362:2477–2512CrossRef
44.
Fitzer EG E, Rozploch F, Steinert D (1987) Application of laser-Raman spectroscopy for characterization of carbon fibres. High Temp High Press 19:537–544
45.
Nasdala L, Pidgeon RT, Wolf D (1996) Heterogeneous metamictization of zircon on a microscale. Geochim Cosmochim Acta 60:1091–1097CrossRef
46.
47.
Grynpas MD (1990) Fluoride effects on bone crystals. J Bone Miner Res 5(suppl 1):S169–S175CrossRef
48.
Posner AS, Eanes ED, Harper RA, Zipkin I (1963) X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch Oral Biol 168:549–570CrossRef
49.
Bang S, Boivin G, Gerster JC, Baud CA (1985) Distribution of fluoride in calcified cartilage of a fluoride-treated osteoporotic patient. Bone (NY) 6:207–210
50.
Fratzl P, Roschger P, Eschberger J, Abendroth B, Klaushofer K (1994) Abnormal bone mineralization after fluoride treatment in osteoporosis: a small-angle X-ray-scattering study. J Bone Miner Res 9:1541–1549CrossRefPubMed
51.
Boivin G, Chavassieux P, Chapuy MC, Baud CA, Meunier PJ (1989) Skeletal fluorosis: histomorphometric analysis of bone changes and bone fluoride content in 29 patients. Bone (NY) 10:89–99
52.
Faibish D, Ott SM, Boskey AL (2006) Mineral changes in osteoporosis: a review. Clin Orthop Relat Res 443:28–38CrossRefPubMed
53.
Landis WJ (1995) The strength of a calcified tissue depends in part on the molecular structure and organization of its constituent mineral crystals in their organic matrix. Bone (NY) 16:533–544
54.
Chachra D, Turner CH, Dunipace AJ, Grynpas MD (1999) The effect of fluoride treatment on bone mineral in rabbits. Calcif Tissue Int 64:345–351CrossRefPubMed
55.
Turner CH, Garetto LP, Dunipace AJ, Zhang W, Wilson ME, Grynpas MD, Chachra D, McClintock R, Peacock M, Stookey GK (1997) Fluoride treatment increased serum IGF-1, bone turnover, and bone mass, but not bone strength, in rabbits. Calcif Tissue Int 61:77–83CrossRefPubMed
56.
Turner CH, Hasegawa K, Zhang W, Wilson M, Li Y, Dunipace AJ (1995) Fluoride reduces bone strength in older rats. J Dent Res 74:1475–1481CrossRefPubMed
57.
Turner CH, Boivin G, Meunier PJ (1993) A mathematical model for fluoride uptake by the skeleton. Calcif Tissue Int 52:130–138CrossRefPubMed
58.
Li Y, Liang C, Slemenda CW, Ji R, Sun S, Cao J, Emsley CL, Ma F, Wu Y, Ying P, Zhang Y, Gao S, Zhang W, Katz BP, Niu S, Cao S, Johnston CC Jr (2001) Effect of long-term exposure to fluoride in drinking water on risks of bone fractures. J Bone Miner Res 16:932–939CrossRefPubMed
59.
Yerramshetty JS, Akkus O (2008) The associations between mineral crystallinity and the mechanical properties of human cortical bone. Bone (NY) 42:476–482
60.
Tadano S, Giri B, Sato T, Fujisaki K, Todoh M (2008) Estimating nanoscale deformation in bone by X-ray diffraction imaging method. J Biomech 41:945–952CrossRefPubMed
61.
Giri B, Tadano S, Fujisaki K, Sasaki N (2009) Deformation of mineral crystals in cortical bone depending on structural anisotropy. Bone (NY) 44:1111–1120
62.
Durchschlag E, Paschalis EP, Zoehrer R, Roschger P, Fratzl P, Recker R, Phipps R, Klaushofer K (2006) Bone material properties in trabecular bone from human iliac crest biopsies after 3- and 5-year treatment with risedronate. J Bone Miner Res 21:1581–1590CrossRefPubMed
63.
Hanschin RG, Stern WB (1995) X-ray diffraction studies on the lattice perfection of human bone apatite (crista iliaca). Bone (NY) 16:355S–363S
Metadata
Title
Mineral maturity and crystallinity index are distinct characteristics of bone mineral
Authors
Delphine Farlay
Gérard Panczer
Christian Rey
Pierre D. Delmas
Georges Boivin
Publication date
01-07-2010
Publisher
Springer Japan
Published in
Journal of Bone and Mineral Metabolism / Issue 4/2010
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI
https://doi.org/10.1007/s00774-009-0146-7

Other articles of this Issue 4/2010

Journal of Bone and Mineral Metabolism 4/2010 Go to the issue

Original Article

Adiposity and genetic admixture, but not race/ethnicity, influence bone mineral content in peripubertal children

Original Article

Cooperative effect of serum 25-hydroxyvitamin D concentration and a polymorphism of transforming growth factor-β1 gene on the prevalence of vertebral fractures in postmenopausal osteoporosis

Original Article

Binding characteristics of the osteoarthritis-associated protein asporin

Original Article

Cortical and trabecular bone microarchitecture and turnover in alcohol-induced chronic pancreatitis: a histomorphometric study

Original Article

Analgesic effect of raloxifene on back and knee pain in postmenopausal women with osteoporosis and/or osteoarthritis

Original Article

Effects of combination treatment with alendronate and vitamin K2 on bone mineral density and strength in ovariectomized mice
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Heart Failure Video

Year in Review: Heart failure and cardiomyopathies

Watch now

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits