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Archives of Virology
Published in: Archives of Virology 4/2024

01-04-2024 | Coronavirus | Brief Report

A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus

Authors: Ji Hye Baek, Yu-Min Lee, Ngoc Duong Vu, Min-Hui Kim, Jincun Zhao, Van Phan Le, Joo Hyuk Cho, Jung-Eun Park

Published in: Archives of Virology | Issue 4/2024

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Abstract

Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.
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Metadata
Title
A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus
Authors
Ji Hye Baek
Yu-Min Lee
Ngoc Duong Vu
Min-Hui Kim
Jincun Zhao
Van Phan Le
Joo Hyuk Cho
Jung-Eun Park
Publication date
01-04-2024
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 4/2024
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-024-06003-9

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