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01-07-2020 | Polymerase Chain Reaction | Brief Report

A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana

Authors: Nadia Martínez-Marrero, Jesús Aarón Avalos-Calleros, Elizabeth Chiquito-Almanza, Jorge Alberto Acosta-Gallegos, Salvador Ambriz-Granados, José Luis Anaya-López, Gerardo Rafael Argüello-Astorga

Published in: Archives of Virology | Issue 7/2020

Abstract

In this work, a begomovirus isolated from a bean plant coinfected with the potyviruses bean common mosaic virus and bean common mosaic necrosis virus was characterized. The three viruses were detected by high-throughput sequencing and assembly of total small RNAs, but the begomovirus-related contigs did not allow precise identification. Molecular analysis based on standard DNA amplification techniques revealed the presence of a single bipartite virus, which is a novel begomovirus according to the current taxonomic criteria. Infectious clones were generated and agroinoculated into Phaseolus vulgaris and Nicotiana benthamiana plants. In all cases, viral DNA-A and DNA-B were detected in new growths, but no symptoms were observed, thus indicating that this virus produces asymptomatic infections in both host species.

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Title: A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana
Authors: Nadia Martínez-Marrero
Jesús Aarón Avalos-Calleros
Elizabeth Chiquito-Almanza
Jorge Alberto Acosta-Gallegos
Salvador Ambriz-Granados
José Luis Anaya-López
Gerardo Rafael Argüello-Astorga
Publication date: 01-07-2020
Publisher: Springer Vienna
Keyword: Polymerase Chain Reaction
Published in: Archives of Virology / Issue 7/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04646-y

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