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01-06-2020 | Brief Report

Production of immunogenic recombinant L1 protein of bovine papillomavirus type 9 causing teat papillomatosis

Authors: Satoko Watanabe, Tomoyuki Shibahara, Kiyohiko Andoh, Shinichi Hatama, Masaji Mase

Published in: Archives of Virology | Issue 6/2020

Abstract

Bovine papillomavirus type 9 (BPV9) is a causative agent of severe teat papillomatosis. Considering the lack of efficient BPV culture methods, recombinant proteins such as virus-like particles developed through genetic engineering may serve as a useful tool for developing effective vaccines against BPV infection. In this study, we successfully produced immunogenic particles composed of recombinant L1 protein of BPV9 (rBPV9-L1), using a baculovirus expression system. rBPV9-L1-immunized mice produced BPV9-specific IgG, which did not cross-react with BPV type 6, which is another causative agent of teat papillomatosis. Hence, immunogenic rBPV9-L1 is potentially applicable as a vaccine candidate for teat papillomatosis.

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Title: Production of immunogenic recombinant L1 protein of bovine papillomavirus type 9 causing teat papillomatosis
Authors: Satoko Watanabe
Tomoyuki Shibahara
Kiyohiko Andoh
Shinichi Hatama
Masaji Mase
Publication date: 01-06-2020
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 6/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04612-8

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