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01-04-2020 | Annotated Sequence Record

Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea

Authors: Wenyi Liu, Du Hai, Fan Mu, Xiaojing Yu, Yingtong Zhao, Bangling He, Jiatao Xie, Daohong Jiang, Huixiang Liu

Published in: Archives of Virology | Issue 4/2020

Abstract

A novel virus, Botryosphaeria dothidea fusarivirus 1 (BdFV1), was isolated from a fungal strain, SDAU11-86 of Botryosphaeria dothidea, and its complete genome sequence was determined. BdFV1 has a single-stranded positive-sense (+ssRNA) genome with 6,179 nucleotides, excluding the poly(A) tail. The genome of BdFV1 contains two putative open reading frames (ORFs). The first ORF encodes a large polyprotein of 1,544 amino acids (aa) with conserved RNA-dependent RNA polymerase and viral helicase domains. The second ORF encodes a putative 481-aa protein with unknown function. Sequence comparisons and phylogenetic analysis suggested that BdFV1 is a novel mycovirus belonging to the newly proposed family “Fusariviridae”. This is the first report of a +ssRNA mycovirus in B. dothidea.

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Title: Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea
Authors: Wenyi Liu
Du Hai
Fan Mu
Xiaojing Yu
Yingtong Zhao
Bangling He
Jiatao Xie
Daohong Jiang
Huixiang Liu
Publication date: 01-04-2020
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04554-1

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