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01-02-2020 | Brief Report

Genetic variability of pig and human rotavirus group A isolates from Slovakia

Authors: S. Salamunova, A. Jackova, T. Csank, R. Mandelik, J. Novotny, Z. Beckova, L. Helmova, S. Vilcek

Published in: Archives of Virology | Issue 2/2020

Abstract

The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.

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Title: Genetic variability of pig and human rotavirus group A isolates from Slovakia
Authors: S. Salamunova
A. Jackova
T. Csank
R. Mandelik
J. Novotny
Z. Beckova
L. Helmova
S. Vilcek
Publication date: 01-02-2020
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 2/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04504-6

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