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Open Access 01-02-2019 | Brief Report

Ectromelia virus induces tubulin cytoskeletal rearrangement in immune cells accompanied by a loss of the microtubule organizing center and increased α-tubulin acetylation

Authors: Lidia Szulc-Dąbrowska, Mateusz Palusiński, Justyna Struzik, Karolina P. Gregorczyk-Zboroch, Felix N. Toka, Ada Schollenberger, Małgorzata Gieryńska

Published in: Archives of Virology | Issue 2/2019

Abstract

Ectromelia virus (ECTV) is an orthopoxvirus that productively replicates in dendritic cells (DCs), but its influence on the microtubule (MT) cytoskeleton in DCs is not known. Here, we show that ECTV infection of primary murine granulocyte-macrophage colony stimulating factor-derived bone marrow cells (GM-BM) downregulates numerous genes engaged in MT cytoskeleton organization and dynamics. In infected cells, the MT cytoskeleton undergoes dramatic rearrangement and relaxation, accompanied by disappearance of the microtubule organizing centre (MTOC) and increased acetylation and stabilization of MTs, which are exploited by progeny virions for intracellular transport. This indicates a strong ability of ECTV to subvert the MT cytoskeleton of highly specialized immune cells.

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Title: Ectromelia virus induces tubulin cytoskeletal rearrangement in immune cells accompanied by a loss of the microtubule organizing center and increased α-tubulin acetylation
Authors: Lidia Szulc-Dąbrowska
Mateusz Palusiński
Justyna Struzik
Karolina P. Gregorczyk-Zboroch
Felix N. Toka
Ada Schollenberger
Małgorzata Gieryńska
Publication date: 01-02-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 2/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-4030-4

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