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01-10-2018 | Brief Report

Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia

Authors: Dan Zhang, Haiyan Mao, Xiuyu Lou, Junhang Pan, Hao Yan, Hongfeng Tang, Yan Shu, Yun Zhao, Xiaoli Cheng, Hong Tao, Yanjun Zhang, Xuejun Ma

Published in: Archives of Virology | Issue 10/2018

Abstract

We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China.

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Title: Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia
Authors: Dan Zhang
Haiyan Mao
Xiuyu Lou
Junhang Pan
Hao Yan
Hongfeng Tang
Yan Shu
Yun Zhao
Xiaoli Cheng
Hong Tao
Yanjun Zhang
Xuejun Ma
Publication date: 01-10-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 10/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3921-8

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