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01-05-2018 | Brief Report

Mutation of I176R in the E coding region weakens Japanese encephalitis virus neurovirulence, but not its growth rate in BHK-21 cells

Authors: Yuyong Zhou, Rui Wu, Qin Zhao, Yung-Fu Chang, Xintian Wen, Yao Feng, Xiaobo Huang, Yiping Wen, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao

Published in: Archives of Virology | Issue 5/2018

Abstract

Previously, we isolated the Japanese encephalitis virus (JEV) strain SCYA201201. In this study, we passed the SCYA201201 strain in Syrian baby hamster kidney (BHK-21) cells 120 times to obtain the SCYA201201-0901 strain, which exhibited an attenuated phenotype in mice. Comparison of SCYA201201-0901 amino acid sequences with those of other JEV strains revealed a single mutation, I176R, in the E coding region. Using reverse genetic technology, we provide evidence that this single E-I176R mutation does not affect virus growth in BHK-21 cells but significantly decreases JEV neurovirulence in mice. This study provides critical information for understanding the molecular mechanism of JEV attenuation.

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Title: Mutation of I176R in the E coding region weakens Japanese encephalitis virus neurovirulence, but not its growth rate in BHK-21 cells
Authors: Yuyong Zhou
Rui Wu
Qin Zhao
Yung-Fu Chang
Xintian Wen
Yao Feng
Xiaobo Huang
Yiping Wen
Qigui Yan
Yong Huang
Xiaoping Ma
Xinfeng Han
Sanjie Cao
Publication date: 01-05-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 5/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3765-2

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