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Open Access 01-03-2019 | Original Article

High frequency and diversity of parechovirus A in a cohort of Malawian children

Authors: Lieke Brouwer, Eveliina Karelehto, Alvin X. Han, Xiomara V. Thomas, Andrea H. L. Bruning, Job C. J. Calis, Michaël Boele van Hensbroek, Brenda M. Westerhuis, Darsha Amarthalingam, Sylvie M. Koekkoek, Sjoerd P. H. Rebers, Kamija S. Phiri, Katja C. Wolthers, Dasja Pajkrt

Published in: Archives of Virology | Issue 3/2019

Abstract

Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Basic Local Alignment Search Tool (BLAST) analysis. PeV was found in 57% of stool samples. Age was significantly associated with PeV positivity (p = 0.01). Typing by phylogenetic analysis resulted in 15 different types, while BLAST typing resulted in 14 different types and several indeterminate strains. In total, six strains showed inconsistencies in typing between the two methods. One strain, P02-4058, remained untypable by all methods, but appeared to belong to the recently reclassified PeV-A19 genotype. PeV-A1, -A2 and -A3 were the most prevalent types (26.8%, 13.8% and 9.8%, respectively). Both the prevalence and genetic diversity found in our study were remarkably high. Our data provide an important contribution to the scarce data available on PeV epidemiology in Africa.

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Title: High frequency and diversity of parechovirus A in a cohort of Malawian children
Authors: Lieke Brouwer
Eveliina Karelehto
Alvin X. Han
Xiomara V. Thomas
Andrea H. L. Bruning
Job C. J. Calis
Michaël Boele van Hensbroek
Brenda M. Westerhuis
Darsha Amarthalingam
Sylvie M. Koekkoek
Sjoerd P. H. Rebers
Kamija S. Phiri
Katja C. Wolthers
Dasja Pajkrt
Publication date: 01-03-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-04131-7

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