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01-04-2018 | Original Article

HPV genotypes detected by linear array and next-generation sequencing in anal samples from HIV positive men who have sex with men in Mexico

Authors: Luz A. González-Hernández, María G. Flores-Miramontes, Adriana Aguilar-Lemarroy, Krissya S. Quintanilla-Peña, Fabiola L. Martin-Amaya-Barajas, Moisés Ramos-Solano, Luis F. Enciso Gómez, Jaime F. Andrade-Villanueva, Luis F. Jave-Suárez

Published in: Archives of Virology | Issue 4/2018

Abstract

The incidence of anal cancer has been rising, especially in HIV+ patients and has been associated with HPV infection. HIV+ patients are more at risk of HPV coinfection and are seven times more likely to have persistent HPV infection; moreover, HIV+ men have an increased risk of developing anal cancer compared to HIV+ women. The development of screening strategies for the detection of HPV in HIV+ men is of major importance; however, there is not enough information about the HPV genotypes and variants that are colonizing the anal epithelia of HIV+ men in diverse geographical regions. Therefore, this work was aimed at identifying HPV genotypes present in the anal epithelium of HIV+ men who have sex with men (MSM), with or without anal lesions (n = 75). For HPV genotyping, two approaches were performed: Linear Array HPV Genotyping Test and next-generation sequencing (NGS). In general, the six most frequent HPV genotypes found by Linear Array were HPV6, 62, 61, 81, 16 and 51. On the other hand, employing NGS, a total of 36 HPV genotypes belonging to both alpha and beta genera were found. The genotypes with the greatest number of reads, according to the diagnostic group, were: HPV81, 45, 6, 51 and 61 in MSM without anal lesions (WAIN); HPV6, 61, 70, 62 and 66 in MSM with atypical lesions (AAL); HPV6, 11, 66, 81 and 61 in MSM with anal intraepithelial neoplasia grade I (AIN I); and HPV16, 81, 58, 61 and 52 with AIN III. Additionally, a great diversity of L1 variants was observed, especially in genotypes HPV16, 58, 61, 52, 45 and 59.

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Title: HPV genotypes detected by linear array and next-generation sequencing in anal samples from HIV positive men who have sex with men in Mexico
Authors: Luz A. González-Hernández
María G. Flores-Miramontes
Adriana Aguilar-Lemarroy
Krissya S. Quintanilla-Peña
Fabiola L. Martin-Amaya-Barajas
Moisés Ramos-Solano
Luis F. Enciso Gómez
Jaime F. Andrade-Villanueva
Luis F. Jave-Suárez
Publication date: 01-04-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3697-2

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