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Archives of Virology
Published in: Archives of Virology 4/2018

01-04-2018 | Original Article

Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture

Authors: Kun Qian, Xiaowei Cheng, Danyang Zhang, Hongxia Shao, Yongxiu Yao, Venugopal Nair, Aijian Qin

Published in: Archives of Virology | Issue 4/2018

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Abstract

Lithium chloride (LiCl) has been reported to possess antiviral activity against several viruses. In the current study, we assessed the antiviral activity effect of LiCl on ALV-J infection in CEF cells by using real-time PCR, Western blot analysis, IFA and p27 ELISA analysis. Our results showed that both viral RNA copy number and protein level decreased significantly in a dose and time dependent manner. Time-course analysis revealed that the antiviral effect was more pronounced when CEFs were treated at the post infection stage rather than at early absorption or pre-absorption stages. Further experiments demonstrated that LiCl did not affect virus attachment or entry, but rather affected early virus replication. We also found that inhibition of viral replication after LiCl treatment was associated with reduced mRNA levels of pro-inflammatory cytokines. These results demonstrate that LiCl effectively blocked ALV-J replication in CEF cells and may be used as an antiviral agent against ALV-J.
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Metadata
Title
Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture
Authors
Kun Qian
Xiaowei Cheng
Danyang Zhang
Hongxia Shao
Yongxiu Yao
Venugopal Nair
Aijian Qin
Publication date
01-04-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 4/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3692-7

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