Top

Published in:

01-03-2018 | Annotated Sequence Record

Genome characterization of an Argentinean isolate of alfalfa leaf curl virus

Authors: Nicolás Bejerman, Verónica Trucco, Soledad de Breuil, Patricia Rodriguez Pardina, Sergio Lenardon, Fabián Giolitti

Published in: Archives of Virology | Issue 3/2018

Abstract

We investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.

Available only for authorised users

Abascal F, Zardoya R, Posada D (2005) ProtTest: selection of best-fit models of protein evolution. Bioinformatics 21:2104–2105CrossRefPubMed

Bejerman N, Nome C, Giolitti F, Kitajima E, de Breuil S, Pérez Fernández J, Basigalup D, Cornacchione M, Lenardon S (2011) First report of a Rhabdovirus infecting alfalfa in Argentina. Plant Dis 95:771CrossRef

Bejerman N, Giolitti F, Trucco V, de Breuil S, Nome C, Lenardon S, Dietzgen R (2015) Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. Virology 483:275–283CrossRefPubMed

Bejerman N, Giolitti F, Trucco V, de Breuil S, Dietzgen R, Lenardon S (2016) Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms. Arch Virol 161:2029–2032CrossRefPubMed

Bernardo P, Golden M, Akram M, Naimuddin Nadarajan N et al (2013) Identification and characterisation of a highly divergent geminivirus: evolutionary and taxonomic implications. Virus Res 177:35–45CrossRefPubMed

Bernardo P, Muhire B, Francois S, Deshoux M, Hartnady P et al (2016) Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virol 493:142–153CrossRef

Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797CrossRefPubMedPubMedCentral

Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis Version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefPubMed

10.

Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003CrossRefPubMedPubMedCentral

11.

Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277CrossRefPubMedPubMedCentral

12.

Roumagnac P, Granier M, Bernardo P, Deshoux M, Ferdinand R et al (2015) Alfalfa leaf curl virus: an aphid-transmitted geminivirus. J Virol 89:9683–9688CrossRefPubMedPubMedCentral

13.

Santorum JM, Darriba D, Taboada GL, Posada D (2014) jmodeltest.org: selection of nucleotide substitution models on the cloud. Bioinformatics 30:1310–1311CrossRefPubMedPubMedCentral

14.

Susi H, Laine A-L, Filloux D, Kraberger S, Farkas K et al (2017) Genome sequences of a capulavirus infecting Plantago lanceolata in the Åland archipelago of Finland. Arch Virol 162:2041–2045CrossRefPubMed

15.

Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2014) Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina. Virus Genes 48:562–565CrossRefPubMed

16.

Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2016) Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields. Eur J Plant Pathol 146:207–212CrossRef

17.

Varsani A, Roumagnac P, Fuchs M, Navas-Castillo J, Moriones E et al (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Arch Virol 162:1819–1831CrossRefPubMed

Title: Genome characterization of an Argentinean isolate of alfalfa leaf curl virus
Authors: Nicolás Bejerman
Verónica Trucco
Soledad de Breuil
Patricia Rodriguez Pardina
Sergio Lenardon
Fabián Giolitti
Publication date: 01-03-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3673-x

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Genome characterization of an Argentinean isolate of alfalfa leaf curl virus

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2018

Characterization of a novel single-stranded RNA mycovirus related to invertebrate viruses from the plant pathogen Verticillium dahliae

Hepatitis C virus infection among illicit drug users in an archipelago of the Amazon

Completed sequence and corrected annotation of the genome of maize Iranian mosaic virus

Significant differences in the intra-host genetic diversity of Helicoverpa armigera nucleopolyhedrovirus dnapol after serial in vivo passages in the same insect population

The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the Ty-1 resistance gene

Pharmacophoric characteristics of dengue virus NS2B/NS3pro inhibitors: a systematic review of the most promising compounds

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page