Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 7/2017

01-07-2017 | Brief Report

Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants

Authors: Yanhong Qiu, Yongjiang Zhang, Fan Hu, Shuifang Zhu

Published in: Archives of Virology | Issue 7/2017

Login to get access

Abstract

This study characterized the viral small interfering RNAs (vsiRNAs) from Nicotiana tabacum cv. Samsun infected with a cucumber mosaic virus (CMV) 2b-deficient mutant. Most vsiRNAs were 21 -22 nucleotides in length and the 5′-terminal ends were dominated by A and U, respectively. The observed vsiRNAs were heterogeneously distributed throughout the CMV genome; however, most of the vsiRNAs were derived from sense strands, as opposed to antisense strands. These results demonstrate the conserved and specific function of Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins in tobacco. Finally, it was revealed that vsiRNAs target abundant host genes, indicating complex roles for CMV vsiRNAs during the development of symptoms.
Literature
1.
Wang MB, Masuta C, Smith NA, Shimura H (2012) RNA silencing and plant viral diseases. Mol Plant Microbe Interact 25:1275–1285CrossRefPubMed
2.
3.
4.
Pattanayak D, Solanke AU, Kumar PA (2013) Plant RNA interference pathways: diversity in function, similarity in action. Plant Mol Biol Rep 31:493–506CrossRef
5.
6.
Wang XB, Wu QF, Ito T, Cillo F, Li WX, Chen XM, Yu JL, Ding SW (2010) RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in Arabidopsis thaliana. PNAS 107:484–489CrossRefPubMed
7.
Sharma N, Sahu PP, Puranik S, Prasad M (2013) Recent Advances in plant-virus interaction with emphasis on small interfering RNAs (siRNAs). Mol Biotechnol 55:63–77CrossRefPubMed
8.
Pumplin N, Voinnet O (2013) RNA silencing suppression by plant pathogens: defence, counter—defence and counter-counter-defence. Nat Rev Microbiol 11:745–760CrossRefPubMed
9.
10.
11.
Shimura H, Pantaleo V, Ishihara T, Myojo N, Inaba J, Sueda K, Burgyan J, Masuta C (2011) A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery. Plos Pathog 7:e1002021CrossRefPubMedPubMedCentral
12.
Shi B, Lin L, Wang S, Guo Q, Zhou H, Rong L, Li J, Peng J, Lu Y, Zheng H, Yang Y, Chen Z, Zhao J, Jiang T, Song B, Chen J, Yan F (2016) Identification and regulation of host genes related to Rice stripe virus symptom production. New Phytol 209:1106–1119CrossRefPubMed
13.
14.
15.
Mochizuki T, Ohki ST (2012) Cucumber mosaic virus: viral genes as virulence determinants. Mol Plant Pathol 13:217–225CrossRefPubMed
16.
Csorba T, Kontra L, Burgyan J (2015) viral silencing suppressors: Tools forged to fine-tune host-pathogen coexistence. Virology 479:85–103CrossRefPubMed
17.
Mochizuki T, Yamazaki R, Wada T, Ohki ST (2014) Coat protein mutations in an attenuated Cucumber mosaic virus encoding mutant 2b protein that lacks RNA silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. Virology 456:292–299CrossRefPubMed
18.
Du ZY, Chen FF, Liao OS, Zhang HR, Cheni YF, Chen JS (2007) 2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species. J Gen Virol 88:2596–2604CrossRefPubMed
19.
20.
Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R (2009) The sequence alignment/map format and SAMtools. Bioinformatics 25:2078–2079CrossRefPubMedPubMedCentral
21.
Lin KY, Cheng CP, Chang BC, Wang WC, Huang YW, Lee YS, Huang HD, Hsu YH, Lin NS (2010) Global analyses of small interfering RNAs derived from Bamboo mosaic virus and its associated satellite RNAs in different plants. PLoS One 5:e11928CrossRefPubMedPubMedCentral
22.
Margaria P, Miozzi L, Ciuffo M, Rosa C, Axtell MJ, Pappu HR, Turina M (2016) Comparison of small RNA profiles in Nicotiana benthamiana and Solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection. Virus Res 211:38–45CrossRefPubMed
23.
Yan F, Zhang H, Adams MJ, Yang J, Peng J, Antoniw JF, Zhou Y, Chen J (2010) Characterization of siRNAs derived from rice stripe virus in infected rice plants by deep sequencing. Arch Virol 155:935–940CrossRef
24.
Li Y, Deng C, Shang Q, Zhao X, Liu X, Zhou Q (2016) Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants. Arch Virol 161:455–458CrossRefPubMed
25.
Chapman EJ, Carrington JC (2007) Specialization and evolution of endogenous small RNA pathways. Nat Rev Genet 8:884–896CrossRefPubMed
26.
Wassenegger M, Krczal G (2006) Nomenclature and functions of RNA-directed RNA polymerases. Trends Plant Sci 11:142–151CrossRefPubMed
27.
28.
Wang XB, Jovel J, Udomporn P, Wang Y, Wu QF, Li WX, Gasciolli V, Vaucheret H, Ding SW (2011) The 21-nucleotide, but not 22-nucleotide, viral secondary small interfering RNAs direct potent antiviral defense by two cooperative argonautes in Arabidopsis thaliana. Plant Cell 23:1625–1638CrossRefPubMedPubMedCentral
29.
Margaria P, Miozzi L, Rosa C, Axtell MJ, Pappu HR, Turina M (2015) Small RNA profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected Nicotiana benthamiana. Virus Res 208:30–38CrossRefPubMed
30.
Xu Y, Huang L, Fu S, Wu J, Zhou X (2012) Population diversity of rice stripe virus-derived siRNAs in three different hosts and RNAi-based antiviral immunity in Laodelphgax striatellus. PLoS One 7:e46238CrossRefPubMedPubMedCentral
31.
Mi S, Cai T, Hu Y, Chen Y, Hodges E, Ni F, Wu L, Li S, Zhou H, Long C, Chen S, Hannon GJ, Qi Y (2008) Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5’ terminal nucleotide. Cell 133:116–127CrossRefPubMedPubMedCentral
32.
Ogwok E, Ilyas M, Alicai T, Rey ME, Taylor NJ (2016) Comparative analysis of virus-derived small RNAs within cassava (Manihot esculenta Crantz) infected with cassava brown streak viruses. Virus Res 215:1–11CrossRefPubMedPubMedCentral
33.
Donaire L, Wang Y, Gonzalez-Ibeas D, Mayer KF, Aranda MA, Llave C (2009) Deep-sequencing of plant viral small RNAs reveals effective and widespread targeting of viral genomes. Virology 392:203–214CrossRefPubMed
34.
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25:25–29CrossRefPubMedPubMedCentral
35.
Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M (2004) The KEGG resource for deciphering the genome. Nucl Acids Res 32:277–280CrossRef
36.
Lu J, Du ZX, Kong J, Chen LN, Qiu YH, Li GF, Meng XH, Zhu SF (2012) Transcriptome analysis of Nicotiana tabacum infected by cucumber mosaic virus during systemic symptom development. PloS One 7:e43447CrossRefPubMedPubMedCentral
Metadata
Title
Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants
Authors
Yanhong Qiu
Yongjiang Zhang
Fan Hu
Shuifang Zhu
Publication date
01-07-2017
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3335-z

Other articles of this Issue 7/2017

Archives of Virology 7/2017 Go to the issue

Original Article

Detection and characterization of hepatitis A virus circulating in Egypt

Annotated Sequence Record

A novel vitivirus-like sequence found in Arracacia xanthorrhiza plants by high throughput sequencing

Original Article

Identification of host factors potentially involved in RTM-mediated resistance during potyvirus long distance movement

Brief Report

An update on the rabbit hemorrhagic disease virus (RHDV) strains circulating in Portugal in the 1990s: earliest detection of G3-G5 and G6

Brief Report

Characterization of lettuce big-vein associated virus and Mirafiori lettuce big-vein virus infecting lettuce in Saudi Arabia

Original Article

The papain-like protease of avian infectious bronchitis virus has deubiquitinating activity
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Heart Failure Video

Year in Review: Heart failure and cardiomyopathies

Watch now

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits