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01-07-2017 | Annotated Sequence Record

Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus

Authors: Kuo-Shiou Huang, Chia-Hsing Tai, Ying-Huey Cheng, Shu-Hui Lin, Tsung-Chi Chen, Fuh-Jyh Jan

Published in: Archives of Virology | Issue 7/2017

Abstract

Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.

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King AMQ, Lefkowitz E, Adams MJ, Carstens EB (2012) Virus taxonomy: 9th report of the international committee on taxonomy of viruses. Elsevier, New York

de Haan P, Kormelink R, de Oliveira Resende R, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 72:2207–2216CrossRefPubMed

Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs. Arch Virol 145:1035–1045CrossRefPubMed

Kormelink R, Storms M, Van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NS_M protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56–65CrossRefPubMed

Storms MMH, Kormelink R, Peters D, Van Lent JWM, Goldbach RW (1995) The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. Virology 214:485–493CrossRefPubMed

Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532:75–79CrossRefPubMed

Bucher E, Sijen T, de Haan P, Goldbach R, Prins M (2003) Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. J Virol 77:1329–1336CrossRefPubMedPubMedCentral

de Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001–1007CrossRefPubMed

Cheng YH, Zheng YX, Tai CH, Yen JH, Chen YK, Jan FJ (2014) Identification, characterisation and detection of a new tospovirus on sweet pepper. Ann Appl Biol 164:107–115CrossRef

10.

Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–289CrossRefPubMedPubMedCentral

11.

Valverde RA, Dodds JA, Heick JA (1986) Double-stranded ribonucleic acid from plants infected with viruses having elongated particles and undivided genomes. Phytopathology 76:459–465CrossRef

12.

Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMed

13.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentral

14.

Petersen TN, Brunak S, von Heijne G, Nielsen H (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 8:785–786CrossRefPubMed

15.

Gupta R, Jung E, Brunak S (2004) Prediction of N-glycosylation sites in human proteins. http://www.cbs.dtu.dk/services/NetNGlyc/

16.

Steentoft C, Vakhrushev SY, Joshi HJ, Kong Y, Vester-Christensen MB, Schjoldager KTBG, Lavrsen K, Dabelsteen S, Pedersen NB, Marcos-Silva L, Gupta R, Paul Bennett E, Mandel U, Brunak S, Wandall HH, Levery SB, Clausen H (2013) Precision mapping of the human O-GalNAc glycoproteome through simple cell technology. EMBO J 32:1478–1488CrossRefPubMedPubMedCentral

17.

Hulo N, Bairoch A, Bulliard V, Cerutti L, De Castro E, Langendijk-Genevaux PS, Pagni M, Sigrist CJA (2006) The PROSITE database. Nucleic Acids Res 34:D227–D230CrossRefPubMed

Title: Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus
Authors: Kuo-Shiou Huang
Chia-Hsing Tai
Ying-Huey Cheng
Shu-Hui Lin
Tsung-Chi Chen
Fuh-Jyh Jan
Publication date: 01-07-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 7/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3260-1

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