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Archives of Virology
Published in: Archives of Virology 2/2017

01-02-2017 | Brief Report

Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus

Authors: Mohammad Amin Almasi, Galavizh Almasi

Published in: Archives of Virology | Issue 2/2017

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Abstract

Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
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Metadata
Title
Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus
Authors
Mohammad Amin Almasi
Galavizh Almasi
Publication date
01-02-2017
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-3116-0

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