Top

Published in:

01-07-2016 | Original Article

Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA

Authors: Sunil K. Mor, Nicholas B. D. Phelps

Published in: Archives of Virology | Issue 7/2016

Abstract

During a survey of apparently healthy baitfish from the state of Minnesota, a novel piscine-myocarditis-like virus (PMCLV) was detected in golden shiners (Notemigonus crysoleucas). The nearly complete genome sequence is 5819 nt long, including a partial 5’ untranslated region (UTR) of 100 nt. The sequence is divided into three ORFs: the complete ORF1 and ORF2, encoding proteins of 818 and 831 amino acids, respectively, and a partial ORF3 encoding 248 amino acids of the corresponding protein. This novel virus sequence was most closely related to piscine myocarditis virus (PMCV), with a 49.0 % and 58.2 % amino acid identity in the ORF1 (YP005481249)- and ORF2 (YP004581250)-encoded proteins, respectively. Six of 56 retail outlets (e.g., bait shops) were positive during the 2014–2015 survey, indicating a 10.7 % prevalence of the novel virus in this population of golden shiners. Currently, there is no disease that is known to be associated with this virus.

Amin AB, Trasti J (1988) Endomyocarditis in Atlantic salmon in Norwegian seafarms: a case report. Bull Eur Assoc Fish Pathol 8:70–71

Brocklebank J, Raverty S (2002) Sudden mortality caused by cardiac deformities following screening of preharvest farmed Atlantic salmon (Salmo salar) and by cardiomyopathy of postintraperitoneally vaccinated Atlanticsalmon parr in British Columbia. Can Vet J 43:129–130PubMedPubMedCentral

Bockerman I, Wiik-Nielsen CR, Sindre H, Johansen R, Tengs T (2011) Prevalence of piscine myocarditis virus (PMCV) in marine fish species. J Fish Dis 34:955–957CrossRefPubMed

Drummond AJ, Ashton B, Buxton S, Cheung M, Copper A, Duran C, Field M, Held J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2011) Geneious v5.4. http://www.geneious.com/

Garseth AH, Biering E, Tengs T (2012) Piscine myocarditis virus (PMCV) in wild Atlantic salmon Salmo salar. Dis Aquat Organ 102:157–161CrossRefPubMed

Goodwin AE, Peterson JE, Meyers TR, Money DJ (2004) Transmission of exotic fish viruses: the relative risks of wild and cultured bait. Fisheries 29:19–23CrossRef

Haugland O, Mikalsen AB, Nilsen P, Lindmo K, Thu BJ, Eliassen TM, Roos N, Rode M, Evensen O (2011) Cardiomyopathy syndrome of Atlantic salmon (Salmo salar L.) is caused by a double-stranded RNA virus of the Totiviridae family. J Virol 85:5275–5286CrossRefPubMedPubMedCentral

Isawa H, Kuwata R, Hoshino K, Tsuda Y, Sakai K, Watanabe S, Nishimura M, Satho T, Kataoka M, Nagata N, Hasegawa H, Bando H, Yano K, Sasaki T, Kobayashi M, Mizutani T, Sawabe K (2011) Identification and molecular characterization of a new nonsegmented double-stranded RNA virus isolated from Culex mosquitoes in Japan. Virus Res 155:147–155CrossRefPubMed

Mor SK, Phelps NBD (2016) Molecular detection of a novel totivirus from golden shiner (Notemigonus crysoleucas) baitfish in the USA. ARVI-D-16-00039R1 (manuscript in preparation)

10.

Naim S, Brown JK, Nibert ML (2014) Genetic diversification of penaeid shrimp infectious myonecrosis virus between Indonesia and Brazil. Virus Res 189:97–105CrossRefPubMed

11.

Phelps NBD, Mor SK, Armien AG, Batts W, Goodwin AE, Hopper L, McCann R, Ng TFF, Puzach C, Waltzek T, Winton J, Goyal SM (2014) Isolation and molecular characterization of a novel picornavirus from baitfish in the USA. PLoS One 9:875–893CrossRef

12.

Poppe TT, Seierstad SL (2003) First description of cardiomyopathy syndrome (CMS)-related lesions in wild Atlantic salmon Salmo salar in Norway. Dis Aquat Organ 56:87–88CrossRefPubMed

13.

Rodger H, Turnbull T (2000) Cardiomyopathy syndrome in farmed Scottish salmon. Vet Rec 146:500–501CrossRefPubMed

14.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentral

15.

Tang J, Ochoa WF, Sinkovits RS, Poulos BT, Ghabrial SA, Lightner DV, Baker TS, Nibert ML (2008) Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes. Proc Natl Acad Sci USA 105:17526–17531CrossRefPubMedPubMedCentral

16.

Tengs T, Bockerman I (2012) A strain of piscine myocarditis virus infecting Atlantic argentine, Argentina silus (Ascanius). J Fish Dis 35:545–547CrossRefPubMed

17.

USDA (United States Department of Agriculture) (2006) Census of aquaculture (2005). 2002 Census of Agriculture: Special Studies Part 2, vol 3 AC-02-SP-2

18.

Wickner RB, Ghabrial SA, Nibert ML, Patterson JL, Wang CC (2012) Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Totiviridae. Academic Press, Waltham, pp 639–650

19.

Wiik-Nielsen J, Alarcón M, Fineid B, Rode M, Haugland Ø (2013) Genetic variation in Norwegian piscine myocarditis virus in Atlantic salmon, Salmo salar L. J Fish Dis 36:129–139CrossRefPubMed

20.

Yang X, Zhang Y, Ge X, Yuan J, Shi Z (2012) A novel totivirus-like virus isolated from bat guano. Arch Virol 157:1093–1099CrossRefPubMed

21.

Zhai Y, Attoui H, Mohd Jaafar F, Wang HQ, Cao YX, Fan SP, Sun YX, Liu LD, Mertens PP, Meng WS, Wang D, Liang G (2010) Isolation and full-length sequence analysis of Armigeres subalbatus totivirus, the first totivirus isolate from mosquitoes representing a proposed novel genus (Artivirus) of the family Totiviridae. J Gen Virol 91:2836–2845CrossRefPubMed

Title: Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA
Authors: Sunil K. Mor
Nicholas B. D. Phelps
Publication date: 01-07-2016
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 7/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2873-0

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 7/2016

Virulence of a novel reassortant canine H3N2 influenza virus in ferret, dog and mouse models

Processing, fusogenicity, virion incorporation and CXCR4-binding activity of a feline immunodeficiency virus envelope glycoprotein lacking the two conserved N-glycosylation sites at the C-terminus of the V3 domain

Bovine viral diarrhea virus structural protein E2 as a complement regulatory protein

Antiviral activity of recombinant porcine surfactant protein A against porcine reproductive and respiratory syndrome virus in vitro

Study of the epidemiology and etiological characteristics of hand, foot, and mouth disease in Suzhou City, East China, 2011–2014

Emergence of reticuloendotheliosis virus in pigeons in Guangdong Province, Southern China

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page