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Archives of Virology
Published in: Archives of Virology 5/2015

01-05-2015 | Brief Report

BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve

Authors: Shin-nosuke Takeshima, Yuri Kitamura-Muramatsu, Yuan Yuan, Meripet Polat, Susumu Saito, Yoko Aida

Published in: Archives of Virology | Issue 5/2015

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Abstract

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the “BLV-CoCoMo-qPCR-2” assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.
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Metadata
Title
BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve
Authors
Shin-nosuke Takeshima
Yuri Kitamura-Muramatsu
Yuan Yuan
Meripet Polat
Susumu Saito
Yoko Aida
Publication date
01-05-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 5/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2377-3

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