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Archives of Virology
Published in: Archives of Virology 1/2015

01-01-2015 | Brief Report

Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses

Authors: D. Cecilia, M. Kakade, K. Alagarasu, J. Patil, A. Salunke, D. Parashar, P. S. Shah

Published in: Archives of Virology | Issue 1/2015

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Abstract

Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect and quantitate RNA of all dengue virus (DENV) serotypes and chikungunya virus (CHIKV). The sensitivity was 100 % for DENV and 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue and chikungunya in Pune, where the viruses co-circulate.
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Metadata
Title
Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses
Authors
D. Cecilia
M. Kakade
K. Alagarasu
J. Patil
A. Salunke
D. Parashar
P. S. Shah
Publication date
01-01-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2217-x

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