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Published in: Archives of Virology 8/2014

01-08-2014 | Brief Report

Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines

Authors: Miroslav Glasa, Lukáš Predajňa, Petr Komínek, Alžbeta Nagyová, Thierry Candresse, Antonio Olmos

Published in: Archives of Virology | Issue 8/2014

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Abstract

Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5′ extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
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Metadata
Title
Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
Authors
Miroslav Glasa
Lukáš Predajňa
Petr Komínek
Alžbeta Nagyová
Thierry Candresse
Antonio Olmos
Publication date
01-08-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2031-5

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