Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 12/2013

01-12-2013 | Brief Report

In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I

Authors: Xianchao Sun, Yong Li, Mengdie Shi, Ning Zhang, Gaixia Wu, Tingting Li, Ling Qing, Changyong Zhou

Published in: Archives of Virology | Issue 12/2013

Login to get access

Abstract

Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells.
Appendix
Available only for authorised users
Literature
1.
Abbink TE, Peart JR, Mos TN, Baulcombe DC, Bol JF, Linthorst HJ (2002) Silencing of a gene encoding a protein component of the oxygen-evolving complex of photosystem II enhances virus replication in plants. Virology 295:307–319PubMedCrossRef
2.
Bao YM, Carter SA, Nelson RS (1996) The 126- and 183-kilodalton proteins of tobacco mosaic virus, and not their common nucleotide sequence, control mosaic symptom formation in tobacco. J Virol 70:6378–6383PubMed
3.
Bhat S, Folimonova SY, Cole AB, Ballard KD, Lei Z, Watson BS, Sumner LW, Nelson RS (2013) Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement. Plant Physiol 161:134–147PubMedCrossRef
4.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRef
5.
Caplan JL, Mamillapalli P, Burch-Smith TM, Czymmek K, Dinesh-Kumar SP (2008) Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell 132:449–462PubMedCrossRef
6.
Deom CM, Oliver MJ, Beachy RN (1987) The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394PubMedCrossRef
7.
Feki S, Loukili MJ, Triki-Marrakchi R, Karimova G, Old I, Ounouna H, Nato A, Nato F, Guesdon JL, Lafaye P, Elgaaied AB (2005) Interaction between tobacco ribulose-1,5-biphosphate carboxylase/oxygenase large subunit (RubisCO-LSU) and the PVY coat protein (PVY-CP). Eur J Plant Pathol 112:221–234CrossRef
8.
Goelet P, Lomonossoff GP, Butler PJ, Akam ME, Gait MJ, Karn J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79:5818–5822PubMedCrossRef
9.
Hodgson RAJ, Beachy RN, Pakrasi HB (1989) Selective-inhibition of photosystem-II in spinach by tobacco mosaic virus—an effect of the viral coat protein. Febs Lett 245:267–270PubMedCrossRef
10.
Knorr DA, Dawson WO (1988) A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity in Nicotiana sylvestris. Proc Natl Acad Sci USA 85:170–174PubMedCrossRef
11.
Lehto K, Tikkanen M, Hiriart JB, Paakkarinen V, Aro EM (2003) Depletion of the photosystem II core complex in mature tobacco leaves infected by the flavum strain of tobacco mosaic virus. Mol Plant Microbe Interact 16:1135–1144PubMedCrossRef
12.
Lewandowski DJ, Dawson WO (2000) Functions of the 126- and 183-kDa proteins of tobacco mosaic virus. Virology 271:90–98PubMedCrossRef
13.
Ma YX, Zhou T, Hong YG, Fan Z, Li H (2008) Decreased level of ferredoxin I in Tobacco mosaic virus-infected tobacco is associated with development of the mosaic symptom. Physiol Mol Plant P 72:39–45CrossRef
14.
McClintock K, Lamarre A, Parsons V, Laliberte JF, Fortin MG (1998) Identification of a 37 kDa plant protein that interacts with the turnip mosaic potyvirus capsid protein using anti-idiotypic-antibodies. Plant Mol Biol 37:197–204PubMedCrossRef
15.
Padmanabhan MS, Dinesh-Kumar SP (2010) All hands on deck—the role of chloroplasts, endoplasmic reticulum, and the nucleus in driving plant innate immunity. Mol Plant Microbe Interact 23:1368–1380PubMedCrossRef
16.
Qiao Y, Li HF, Wong SM, Fan ZF (2009) Plastocyanin transit peptide interacts with potato virus X coat protein, while silencing of plastocyanin reduces coat protein accumulation in chloroplasts and symptom severity in host plants. Mol Plant Microbe Interact 22:1523–1534PubMedCrossRef
17.
Saito T, Yamanaka K, Okada Y (1990) Long-distance movement and viral assembly of tobacco mosaic virus mutants. Virology 176:329–336PubMedCrossRef
18.
Shintaku MH, Carter SA, Bao YM, Nelson RS (1996) Mapping nucleotides in the 126-kDa protein gene that control the differential symptoms induced by two strains of tobacco mosaic virus. Virology 221:218–225PubMedCrossRef
19.
Wang D, Maule AJ (1995) Inhibition of host gene expression associated with plant virus replication. Science 267:229–231PubMedCrossRef
20.
Yang G, Liu XG, Qiu BS (2000) Complete nucleotide sequences and genome structure of two Chinese tobacco mosaic virus isolates deduced from full-length infectious cDNA clones. Chin J Biotechnol 16:437–442
Metadata
Title
In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I
Authors
Xianchao Sun
Yong Li
Mengdie Shi
Ning Zhang
Gaixia Wu
Tingting Li
Ling Qing
Changyong Zhou
Publication date
01-12-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 12/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1778-4

Other articles of this Issue 12/2013

Archives of Virology 12/2013 Go to the issue

Original Article

Matrix metalloproteinases and their tissue inhibitors in serum and cerebrospinal fluid of children with Japanese encephalitis virus infection

Original Article

Genetic diversity and mutation of avian paramyxovirus serotype 1 (Newcastle disease virus) in wild birds and evidence for intercontinental spread

Original Article

Comparison of genomic and amino acid sequences of eight Japanese encephalitis virus isolates from bats

Original Article

Factors responsible for pathogenicity in chickens of a low-pathogenic H7N7 avian influenza virus isolated from a feral duck

Erratum

Erratum to: A confirmation of sapovirus re-infection gastroenteritis cases with different genogroups and genetic shifts in the evolving sapovirus genotypes, 2002-2011

Annotated Sequence Record

Complete nucleotide sequence of Rosa rugosa leaf distortion virus, a new member of the family Tombusviridae
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits