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01-07-2013 | Original Article

A consensus envelope protein domain III can induce neutralizing antibody responses against serotype 2 of dengue virus in non-human primates

Authors: Hsin-Wei Chen, Shih-Jen Liu, Yi-Shiuan Li, Hsueh-Hung Liu, Jy-Ping Tsai, Chen-Yi Chiang, Mei-Yu Chen, Chyi-Sing Hwang, Chin-Cheng Huang, Hui-Mei Hu, Han-Hsuan Chung, Sze-Hsien Wu, Pele Chong, Chih-Hsiang Leng, Chien-Hsiung Pan

Published in: Archives of Virology | Issue 7/2013

Abstract

We have previously demonstrated that vaccination with a subunit dengue vaccine containing a consensus envelope domain III with aluminum phosphate elicits neutralizing antibodies against all four serotypes of dengue virus in mice. In this study, we evaluated the immunogenicity of the subunit dengue vaccine in non-human primates. After vaccination, monkeys that received the subunit vaccine with aluminum phosphate developed a significantly strong and long-lasting antibody response. A specific T cell response with cytokine production was also induced, and this correlated with the antibody response. Additionally, neutralizing antibodies against serotype 2 were detected in two of three monkeys. The increase in serotype-2-specific antibody titers and avidity observed in these two monkeys suggested that a serotype-2-biased antibody response occurs. These data provide evidence that a protective neutralizing antibody response was successfully elicited in non-human primates by the dengue subunit vaccine with aluminum phosphate adjuvant.

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Title: A consensus envelope protein domain III can induce neutralizing antibody responses against serotype 2 of dengue virus in non-human primates
Authors: Hsin-Wei Chen
Shih-Jen Liu
Yi-Shiuan Li
Hsueh-Hung Liu
Jy-Ping Tsai
Chen-Yi Chiang
Mei-Yu Chen
Chyi-Sing Hwang
Chin-Cheng Huang
Hui-Mei Hu
Han-Hsuan Chung
Sze-Hsien Wu
Pele Chong
Chih-Hsiang Leng
Chien-Hsiung Pan
Publication date: 01-07-2013
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 7/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1639-1

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