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Archives of Virology
Published in: Archives of Virology 7/2013

01-07-2013 | Brief Report

Establishment of an agroinoculation system for broad bean wilt virus 2

Authors: Go Atsumi, Reiko Tomita, Kappei Kobayashi, Ken-Taro Sekine

Published in: Archives of Virology | Issue 7/2013

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Abstract

We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5’ end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
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Metadata
Title
Establishment of an agroinoculation system for broad bean wilt virus 2
Authors
Go Atsumi
Reiko Tomita
Kappei Kobayashi
Ken-Taro Sekine
Publication date
01-07-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1625-7

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