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Archives of Virology
Published in: Archives of Virology 11/2012

Open Access 01-11-2012 | Original Article

Functional identification of the DNA packaging terminase from Pseudomonas aeruginosa phage PaP3

Authors: Xiaodong Shen, Ming Li, Yijun Zeng, Xiaomei Hu, Yinling Tan, Xiancai Rao, Xiaolin Jin, Shu Li, Junmin Zhu, Kebin Zhang, Fuquan Hu

Published in: Archives of Virology | Issue 11/2012

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Abstract

Terminase proteins are responsible for DNA recognition and initiation of DNA packaging in phages. We previously reported the genomic sequence of a temperate Pseudomonas aeruginosa phage, PaP3, and determined its precise integration site in the host bacterial chromosome. In this study, we present a detailed functional identification of the DNA packaging terminase for phage PaP3. The purified large subunit p03 was demonstrated to possess ATPase and nuclease activities, as well as the ability to bind to specific DNA when it is unassembled. In addition, a small terminase subunit (p01) of a new type was found and shown to bind specifically to cos-containing DNA and stimulate the cos-cleavage and ATPase activities of p03. The results presented here suggest that PaP3 utilizes a typical cos site mechanism for DNA packaging and provide a first step towards understanding the molecular mechanism of the PaP3 DNA packaging reaction.
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Metadata
Title
Functional identification of the DNA packaging terminase from Pseudomonas aeruginosa phage PaP3
Authors
Xiaodong Shen
Ming Li
Yijun Zeng
Xiaomei Hu
Yinling Tan
Xiancai Rao
Xiaolin Jin
Shu Li
Junmin Zhu
Kebin Zhang
Fuquan Hu
Publication date
01-11-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1409-5

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