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Archives of Virology
Published in: Archives of Virology 8/2012

01-08-2012 | Original Article

RNA silencing suppressor Pns11 of rice gall dwarf virus induces virus-like symptoms in transgenic rice

Authors: Wen-Jin Shen, Xiao-Lei Ruan, Xin-Shen Li, Qin Zhao, Hua-Ping Li

Published in: Archives of Virology | Issue 8/2012

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Abstract

Transgenic rice (Oryza sativa) plants expressing the Pns11 protein of rice gall dwarf virus (RGDV) displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in RGDV-infected rice. Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microRNA160, microRNA162, microRNA167, microRNA168, and the microRNA target OsARF8. Especially, the striking dwarfing phenotype depended on the high expression level of microRNA167. By analogy to other categories of plant viruses, the RNA silencing suppressors encoded by plant dsRNA viruses function as pathogenicity determinants. These findings significantly deepen our current mechanistic understanding of the RNA silencing suppressor (VSR) encoded by a dsRNA virus and provide additional evidence that interference with microRNA expression is a VSR function utilized by a diverse range of viruses.
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Metadata
Title
RNA silencing suppressor Pns11 of rice gall dwarf virus induces virus-like symptoms in transgenic rice
Authors
Wen-Jin Shen
Xiao-Lei Ruan
Xin-Shen Li
Qin Zhao
Hua-Ping Li
Publication date
01-08-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1339-2

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