Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 8/2012

01-08-2012 | Brief Report

Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization

Authors: Yoshihiro Kaku, Akira Noguchi, Glenn A. Marsh, Jennifer A. Barr, Akiko Okutani, Kozue Hotta, Boldbaatar Bazartseren, Christopher C. Broder, Akio Yamada, Satoshi Inoue, Lin-Fa Wang

Published in: Archives of Virology | Issue 8/2012

Login to get access

Abstract

A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 104 TCID50. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.
Literature
1.
Bossart KN, Broder CC (2006) Developments towards effective treatments for Nipah and Hendra virus infection. Expert Rev Anti Infect Ther 4:43–55PubMedCrossRef
2.
Chiang C-F, Lo MK, Rota PA, Spiropoulou CF, Rollin PE (2010) Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA. Virol J 7:115PubMedCrossRef
3.
Daniels P, Ksiazek T, Eaton BT (2001) Laboratory diagnosis of Nipah and Hendra virus infections. Microbes Infect 3:289–295PubMedCrossRef
4.
Drexler JF, Corman VM, Gloza-Rausch F, Seebens A, Annan A, Ipsen A, Kruppa T, Müller MA, Kalko EKV, Adu-Sarkodie Y, Oppong S, Drosten C (2009) Henipavirus RNA in African bats. PLoS ONE 4:e6367PubMedCrossRef
5.
Eaton BT, Broder CC, Middleton D, Wang LF (2006) Hendra and Nipah viruses: different and dangerous. Nat Rev Microbiol 4:23–35PubMedCrossRef
6.
Feldman KS, Foord A, Heine HG, Smith IL, Boyd V, Marsh GA, Wood JLN, Cunningham AA, Wang LF (2009) Design and evaluation of consensus PCR assays for henipaviruses. J Virol Methods 161:52–57PubMedCrossRef
7.
Kaku Y (2009) Nipah virus infection–zoonosis among wild animals, domestic animals and humans. J Disaster Res 4:309–314
8.
Kaku Y, Noguchi A, Marsh GA, McEachern JA, Okutani A, Hotta K, Bazartseren B, Fukushi S, Broder CC, Yamada A, Inoue S, Wang LF (2009) A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein. J Virol Methods 160:7–13PubMedCrossRef
9.
10.
Li Y, Wang J, Hickey AC, Zhang Y, Wu Y, Zhang H, Yuan J, Han Z, McEachern J, Broder CC, Wang LF, Shi Z (2008) Antibodies to Nipah or Nipah-like viruses in bats, China. Emerg Infect Dis 14:1974–1976PubMedCrossRef
11.
Lo MK, Lowe L, Hummel KB, Sazzad HM, Gurley ES, Hossain MJ, Luby SP, Miller DM, Comer JA, Rollin PE, Bellini WJ, Rota PA (2012) Characterization of Nipah virus from outbreaks in Bangladesh, 2008-2010. Emerg Infect Dis 18:248–255PubMedCrossRef
12.
Maisner A, Neufeld J, Weingartl H (2009) Organ- and endotheliotropism of Nipah virus infections in vivo and in vitro. Thromb Haemost 102:1014–1023PubMed
13.
Marsh GA, Todd S, Foord A, Hansson E, Davies K, Wright L, Morrissy C, Halpin K, Middleton D, Field HE, Daniels P, Wang LF (2010) Genome sequence conservation of Hendra virus isolates during spillover to horses, Australia. Emerg Infect Dis 16:1767–1769PubMed
14.
Patch JR, Crameri G, Wang L-F, Eaton BT, Broder CC (2007) Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix protein. Virol J 4:1PubMedCrossRef
15.
Smith I, Broos A, de Jong C, Zeddeman A, Smith C, Smith G, Moore F, Barr J, Crameri G, Marsh G, Tachedjian M, Yu M, Kung YH, Wang L-F, Field H (2011) Identifying hendra virus diversity in pteropid bats. PLoS ONE 6:e25275PubMedCrossRef
16.
Smith IL, Halpin K, Warrilow D, Smith GA (2001) Development of a fluorogenic RT-PCR assay (TaqMan) for the detection of Hendra virus. J Virol Methods 98:33–40PubMedCrossRef
Metadata
Title
Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization
Authors
Yoshihiro Kaku
Akira Noguchi
Glenn A. Marsh
Jennifer A. Barr
Akiko Okutani
Kozue Hotta
Boldbaatar Bazartseren
Christopher C. Broder
Akio Yamada
Satoshi Inoue
Lin-Fa Wang
Publication date
01-08-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1338-3

Other articles of this Issue 8/2012

Archives of Virology 8/2012 Go to the issue

Original Article

Development of a loop-mediated isothermal amplification method for rapid detection of caprine arthritis-encephalitis virus proviral DNA

Brief Report

Correlation of viral load with bone marrow and hematological changes in pale patients with chronic hepatitis C virus

Original Article

Phylogenetic analysis of rabbit hemorrhagic disease virus in China and the antigenic variation of new strains

Brief Report

Isolation and characterization of a Chinese strain of human enterovirus 74 from a healthy child in the Tibet Autonomous Region of China

Original Article

HBV/D1: a major HBV subgenotype circulating in Uyghur patients with chronic HBV infection in Xinjiang, China

Virology Division News

Tepovirus, a novel genus in the family Betaflexiviridae
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits