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Archives of Virology
Published in: Archives of Virology 2/2012

01-02-2012 | Original Article

Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples

Authors: Stephen J. Wylie, Hao Luo, Hua Li, Michael G. K. Jones

Published in: Archives of Virology | Issue 2/2012

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Abstract

RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus, Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.
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Metadata
Title
Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples
Authors
Stephen J. Wylie
Hao Luo
Hua Li
Michael G. K. Jones
Publication date
01-02-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-1166-x

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