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Archives of Virology
Published in: Archives of Virology 8/2011

01-08-2011 | Original Article

The RGD motif in VP31 of white spot syndrome virus is involved in cell adhesion

Authors: Li Li, Zhaoyu Lin, Limei Xu, Feng Yang

Published in: Archives of Virology | Issue 8/2011

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Abstract

Two WSSV envelope proteins, VP31 and VP33, contain a conserved Arg-Gly-Asp (RGD) sequence. In order to investigate the role of the RGD motif, wild-type and RGD-mutated VP31 and VP33 were recombinantly expressed in E. coli. The cell adhesion ability of the proteins was investigated in crayfish haemocytes using a fluorescence assay. The results showed that recombinant wild-type VP31 and VP33 had cell adhesion activity, and the RGD motif in VP31 was required for cell adhesion, which could be inhibited by an RGDT peptide. In contrast, the interaction of VP33 with cells did not require the RGD motif. These data indicate that the RGD motif plays an important role in the interaction between VP31 and host cells.
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Metadata
Title
The RGD motif in VP31 of white spot syndrome virus is involved in cell adhesion
Authors
Li Li
Zhaoyu Lin
Limei Xu
Feng Yang
Publication date
01-08-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-0984-1

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