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Published in: Archives of Virology 2/2011

01-02-2011 | Annotated Sequence Record

Complete genome sequence of the first endornavirus from the ascocarp of the ectomycorrhizal fungus Tuber aestivum Vittad.

Authors: Benjamin Stielow, Hans-Peter Klenk, Wulf Menzel

Published in: Archives of Virology | Issue 2/2011

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Excerpt

The genus Endornavirus was recently approved by the International Committee on Taxonomy of Viruses (ICTV) [1, 10] to be separated from the family Partitiviridae, and endornaviruses have only been described in plants and fungi [4, 15]. They share common properties such as symptom-free infection of their host, occurrence in low-copy number in host cells, lack of true virions [7], and an efficient vertical transmission in their hosts [16]. Endornaviruses have unique plasmid-like properties that differ markedly from those of other conventionally encapsidated viruses [5, 12, 16]. The genomes of endornaviruses consist of linear double-stranded RNA (dsRNA) with a characteristic, single open reading frame (ORF) of up to 18 kb in length, often preceded by a site-specific nick at the 5’ end [5, 7, 8, 12, 18]. The predicted protein from this ORF harbours in its N-terminal part characteristic amino acid (aa) sequence motifs for viral RNA helicases (VHel) or viral methyltransferases (VMet), while aa sequence motifs for UDP-glycosyltransferases (UGT) and RNA-dependent RNA polymerases (RdRp) are located at close proximity to the 3′ end [7]. The remaining polyprotein is unrelated to any known functional proteins in the database. Currently, the INSDC databases contain complete sequences of seven endornaviruses. Three are plant-associated viruses infecting cultivated and wild rice (Oryza sativa endornavirus [OsEV] and Oryza rufipogon endornavirus [OrEV]) [5, 11], respectively), as well as broad bean (Vicia faba endornavirus [VFV]) [13]. In addition, four fungal isolates are known. One isolate each was reported from the mycelia of the violet root rot fungus (Helicobasidium mompa endornavirus 1-670 [HmEV1-670]) [12] and from an informally designated plant-pathogenic oomycete Phytophtora spp. (Phytophtora endornavirus 1 [PEV1]) [6], while two isolates originate from the pathogenic ascomycete Gremmeniella abietina (Gremmeniella abietina type B RNA virus) [15]. Here, we report the complete nucleotide sequence of an isolate of a putatively novel species in the genus Endornavirus from an ectomycorrhizal fungus, isolated from the hypogeous ascomata of a black truffle (Tuber aestivum Vittad.). Accordingly, we propose the name Tuber aestivum endornavirus (TaEV). The TaEV genome structure is highly similar to that of other Ascomycetes-infecting endornaviruses, such as Gremmeniella abietina type B RNA virus [15], containing characteristic motifs for the DEAD-like helicase (DEXDc), VMet and RdRp. …
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Metadata
Title
Complete genome sequence of the first endornavirus from the ascocarp of the ectomycorrhizal fungus Tuber aestivum Vittad.
Authors
Benjamin Stielow
Hans-Peter Klenk
Wulf Menzel
Publication date
01-02-2011
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0875-x

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