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01-04-2011 | Brief Report

Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

Authors: Rui Wu, Yongxiang Tian, Junhua Deng, Keli Yang, Wangwang Liang, Rui Guo, Zhengying Duan, Zewen Liu, Danna Zhou, Diping Xu

Published in: Archives of Virology | Issue 4/2011

Abstract

In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.

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Title: Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice
Authors: Rui Wu
Yongxiang Tian
Junhua Deng
Keli Yang
Wangwang Liang
Rui Guo
Zhengying Duan
Zewen Liu
Danna Zhou
Diping Xu
Publication date: 01-04-2011
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0871-1

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