Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

ACC 2024 Congress

Catch up on all the top stories and latest evidence in cardiology research with our ACC 2024 Congress hub page.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 10/2010

01-10-2010 | Brief Report

Rapid detection of tobacco mosaic virus using the reverse transcription loop-mediated isothermal amplification method

Authors: Yanhua Liu, Zhide Wang, Yumei Qian, Jianmin Mu, Lili Shen, Fenglong Wang, Jinguang Yang

Published in: Archives of Virology | Issue 10/2010

Login to get access

Abstract

Tobacco mosaic virus (TMV) can cause a severe disease that is capable of greatly reducing tobacco quality and yield. In this study, a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of TMV. The concentration of Mg2+, reaction temperature and reaction time of the RT-LAMP were optimized to 5 mM, 65°C, and 60 min, respectively. The detection limit of the method was 100 times higher than that of RT-PCR. Visual inspection of RT-LAMP amplification demonstrated that positive and negative reactions exhibit distinctly different colours in daylight. Our results demonstrate that the method is stable, sensitive and specific.
Literature
1.
Zhu XC, Wang YT, Wang ZF (2002) Tobacco diseases of China. China Agricultural Press, Beijing
2.
Wang JH, Wang YQ, Ji XC (2005) Advances on techniques for detection of plant viruses. Chin J Trop Agric 25(3):71–75
3.
Sun ZF, Hu CQ, Ren CH, Shen Q (2006) Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification. J Virol Methods 131(1):41–46CrossRefPubMed
4.
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):e63CrossRefPubMed
5.
Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229CrossRefPubMed
6.
Fukuta S, Iida T, Mizukami Y, Ishida A, Ueda J, Kanbe M (2003) Detection of Japanese yam mosaic virus by RT-LAMP. Arch Virol 148:1713–1720CrossRefPubMed
7.
Wang Y, Lan QK, Zhao X (2009) Development and application of loop-mediated isothermal amplification for detection of genetically modified crops. Sci Agric Sin 42(4):1473–1477
8.
Yang JG, Zhang S, Shen LL, Qian YM, Chen DX, Wang CS, Huang J, Wang FL (2010) Establish and application of multiple RT-PCR for detection of TMV, CMV and PVY in tobacco. Acta Tabacaria Sin 16(4):80–84
9.
Blomström AL, Hakhverdyan M, Reid SM, Dukes JP, King DP, Belák S, Berg M (2008) A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. J Virol Methods 147(1):188–193CrossRefPubMed
10.
Soliman H, El-Matbouli M (2006) Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet Microbiol 114(3–4):205–213CrossRefPubMed
11.
Li Q, Cy Xue, Qin JP, Zhou QF, Chen F, Bi YZ, Cao YC (2009) An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus. Arch Virol 154:1433–1440CrossRefPubMed
12.
Gunimaladevi I, Kono T, Lapatra SE, Sakai M (2005) A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). Arch Virol 150(5):899–909CrossRefPubMed
13.
Kono T, Savan R, Sakai M, Itami T (2004) Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. J Virol Methods 115:59–65CrossRefPubMed
14.
Teng PH, Chen CL, Sung PF, Lee FC, Ou BR, Lee PY (2007) Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization. J Virol Methods 146(1–2):317–326CrossRefPubMed
15.
Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154CrossRefPubMed
Metadata
Title
Rapid detection of tobacco mosaic virus using the reverse transcription loop-mediated isothermal amplification method
Authors
Yanhua Liu
Zhide Wang
Yumei Qian
Jianmin Mu
Lili Shen
Fenglong Wang
Jinguang Yang
Publication date
01-10-2010
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0746-5

Other articles of this Issue 10/2010

Archives of Virology 10/2010 Go to the issue

Annotated Sequence Record

Molecular variation of hop mosaic virus isolates

Original Article

Analysis of Newcastle disease virus quasispecies and factors affecting the emergence of virulent virus

Annotated Sequence Record

Complete nucleotide sequence of the envelope gene of pseudocowpox virus isolates from Indian dromedaries (Camelus dromedarius)

Original Article

Characterization of Rhynchosia yellow mosaic Yucatan virus, a new recombinant begomovirus associated with two fabaceous weeds in Yucatan, Mexico

Brief Report

Molecular characterization of enteroviruses detected in Gyeong-Ju and Po-Hang provinces of Korea in 2003

Brief Review

The SPO1-related bacteriophages

ACC 2024 Congress Coverage

Cardiology Video

Highlights from the ACC 2024 Congress

Watch now

Watch official videos from the ACC congress summarizing key highlights from the last year in heart failure, cardiomyopathies, valvular disease, pulmonary vascular disease, and pediatric cardiology.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits