Top

Published in:

01-08-2010 | Brief Review

Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein

Authors: Yan Jiang, Yonghuang Luo, Frank Michel, Robert J. Hogan, Ying He, Zhen F. Fu

Published in: Archives of Virology | Issue 8/2010

Abstract

Three anti-rabies virus (RABV) nucleoprotein (N) monoclonal antibodies (Mab) were characterized by immunofluorescence assays, western blotting, and immunohistochemistry. One of these Mabs recognized the antigen by all of the assays, while the other two recognized N only in the native form in the immunofluorescence assay. These data, together with epitope mapping studies, suggest that two anti-N Mabs recognize conformational epitopes located within the N-terminal region of the RABV N protein. The availability of Mabs specific for both linear and epitope-specific antibodies should prove valuable for rabies diagnosis as well as for RABV N protein structure–function studies.

Anzai J, Takamatsu F, Takeuchi K, Kohno T, Morimoto K, Goto H, Minamoto N, Kawai A (1997) Identification of a phosphatase-sensitive epitope of rabies virus nucleoportein which is recognized by a monoclonal antibody 5-2-26. Microbiol Immunol 41:229–240

Blenden D, Creech W, Torres-Anje M (1986) Use of immunofluorescence examination to detect rabies virus antigen in the skin of humans with clinical encephalitis. J Infect Dis 154:698–701PubMed

Dietzschold B, Wang HH, Rupprecht CE, Celis E, Tollis M, Ertl H, Heber-Katz E, Koprowski H (1987) Induction of protective immunity against rabies by immunization with rabies virus ribonucleoprotein. Proc Natl Acad Sci USA 84:9165–9169CrossRefPubMed

Dietzschold B, Rupprecht CE, Tollis M, Lafon M, Mattei J, Wiktor TJ, Koprowski H (1988) Antigenic diversity of the glycoprotein and nucleocapsid proteins of rabies and rabies-related viruses: implications for epidemiology and control of rabies. Rev Infect Dis 10(suppl 4):S785–S798PubMed

Dietzschold B, Morimoto K, Hooper DC, Smith JS, Rupprecht CE, Koprowski H (2000) Genotypic and phenotypic diversity of rabies virus variants involved in human rabies: implications for postexposure prophylaxis. J Human Virol 3:50–57

Fekadu M, Greer PW, Chandler FW, Sanderlin DW (1988) Use of the avidin-biotin peroxidase system to detect rabies antigen in formalin-fixed paraffin-embedded tissues. J Virol Methods 19:91–96CrossRefPubMed

Flamand A, Wiktor TJ, Koprowski H (1980) Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein. J Gen Virol 48:97–104CrossRefPubMed

Fu ZF, Dietzschold B, Schumacher CL, Wunner WH, Ertl HC, Koprowski H (1991) Rabies virus nucleoprotein expressed in and purified from insect cells is efficacious as a vaccine. Proc Natl Acad Sci USA 88:2001–2005CrossRefPubMed

Fu ZF, Wunner WH, Dietzschold B (1994) Immunoprotection by rabies virus nucleoprotein. Curr Top Microbiol Immunol 187:161–172PubMed

10.

Fuerst TR, Niles EG, Studier FW, Moss B (1986) Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 83:8122–8126CrossRefPubMed

11.

Hamir AN, Moser G, Fu ZF, Dietzschold B, Rupprecht CE (1995) Immunohistochemical test for rabies: identification of a diagnostically superior monoclonal antibody. Vet Rec 136:295–296PubMed

12.

Kawai A, Anzai J, Honda Y, Morimoto K, Takeuchi K, Kohno T, Wakisaka K, Goto H, Minamoto N (1997) Monoclonal antibody #5-2-26 recognizes the phosphatase-sensitive epitope of rabies virus nucleoprotein. Microbiol Immunol 41:33–42PubMed

13.

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMed

14.

Lembo T, Niezgoda M, Velasco-Villa A, Cleaveland S, Ernest E, Rupprecht CE (2006) Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis. Emerg Infect Dis 12:310–313PubMed

15.

Madhusudana SN, Shamsundar R, Seetharaman S (2004) In vitro inactivation of the rabies virus by ascorbic acid. Int J Infect Dis 8:21–25CrossRefPubMed

16.

Minamoto N, Tanaka H, Hishida M, Goto H, Ito H, Naruse S, Yamamoto K, Sugiyama M, Kinjo T, Mannen K et al (1994) Linear and conformation-dependent antigenic sites on the nucleoprotein of rabies virus. Microbiol Immunol 38:449–455PubMed

17.

Morimoto K, Hooper DC, Carbaugh H, Fu ZF, Koprowski H, Dietzschold B (1998) Rabies virus quasispecies: implications for pathogenesis. Proc Natl Acad Sci USA 95:3152–3156CrossRefPubMed

18.

Rose J, Whitt M (2000) Rhabdoviridae: the viruses and their replication, 4th edn. Lippincott-Reven, Philadelphia

19.

Schnell MJ, Mebatsion T, Conzelmann KK (1994) Infectious rabies viruses from cloned cDNA. EMBO J 13:4195–4203PubMed

20.

Smith A, Yager P, Baer G (1996) A rapid reproducible test for determining rabies neutralizing antibody In: Meslin FX, Kaplan MM, Koprowski H (eds) Laboratory techniques in rabies. World Health Organization, Geneva, pp 181–192

21.

Toriumi H, Kawai A (2005) Structural difference recognized by a monoclonal antibody #404-11 between the rabies virus nucleocapsid (NC) produced in virus infected cells and the NC-like structures produced in the nucleoprotein (N) cDNA-transfected cells. Microbiol Immunol 49:757–770PubMed

22.

Trimarchi C, Debbie JG (1991) The fluorescent antibody in rabies. In: Baer G (ed) The natural history of rabies viruses. CRC Press, Boca Raton, pp 220–229

23.

Wu X, Gong X, Foley HD, Schnell MJ, Fu ZF (2002) Both viral transcription and replication are reduced when the rabies virus nucleoprotein is not phosphorylated. J Virol 76:4153–4161CrossRefPubMed

24.

Wunner WH, Larson JK, Dietzschold B, Smith CL (1988) The molecular biology of rabies viruses. Rev Infect Dis 10(suppl 4):S771–S784PubMed

25.

Yan X, Prosniak M, Curtis MT, Weiss ML, Faber M, Dietzschold B, Fu ZF (2001) Silver-haired bat rabies virus variant does not induce apoptosis in the brain of experimentally infected mice. J NeuroVirol 7:518–527CrossRefPubMed

26.

Yang J, Hooper DC, Wunner WH, Koprowski H, Dietzschold B, Fu ZF (1998) The specificity of rabies virus RNA encapsidation by nucleoprotein. Virology 242:107–117CrossRefPubMed

27.

Zhang Y, Xiong C, Kuzmin I, Niezgoda M, Fu Z, Rupprecht C (2008) Investigation of the role of healthy dogs as carriers of rabies virus. Vector-Borne Zoon Dis 8:313–320CrossRef

Title: Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein
Authors: Yan Jiang
Yonghuang Luo
Frank Michel
Robert J. Hogan
Ying He
Zhen F. Fu
Publication date: 01-08-2010
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 8/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-010-0709-x

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Characterization of conformation-specific monoclonal antibodies against rabies virus nucleoprotein

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 8/2010

Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication

High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China

The amino terminus of the salmonid alphavirus capsid protein determines subcellular localization and inhibits cellular proliferation

Decreased CD4 and wide-ranging expression of other immune receptors after HIV-envelope-mediated formation of syncytia in vitro

Molecular characterization of Sunflower chlorotic mottle virus: a member of a distinct species in the genus Potyvirus

Xi River virus, a new bat reovirus isolated in southern China

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page