Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 7/2009

01-07-2009 | Original Article

Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals

Authors: Sheila Cabezas, Gertrudis Rojas, Alequis Pavon, Lidice Bernardo, Yinet Castellanos, Mayling Alvarez, Maritza Pupo, Gerardo Guillen, Maria G. Guzman

Published in: Archives of Virology | Issue 7/2009

Login to get access

Abstract

The current study shows the usefulness of dengue-3- and dengue-4-specific phage-displayed antibody fragments as tools for viral detection and serotyping in sera from infected individuals. C6/36 HT cells were inoculated with acute-phase sera from patients, and supernatants were collected daily and analyzed by ELISA using phage-displayed antibody fragments as serotype-specific detector reagents. Serotyping of most samples was possible as early as two to three days postinoculation. Results were comparable with those obtained by indirect immunofluorescence assay but were obtained in a shorter period of time (<1 week). Phage-displayed antibody fragments were better tools for diagnosis and serotyping than their soluble counterparts. Our approach combines the advantages of viral isolation and ELISA techniques. These results could be the basis for the development of a high-throughput method for identifying dengue virus serotypes, which is crucial for the management and control of the disease.
Literature
1.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M (2002) Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40:376–381PubMedCrossRef
2.
Alvarez M, Rodriguez-Roche R, Bernardo L, Morier L, Guzman G (2005) Improved dengue virus plaque formation on BHK21 and LLCMK2 cells: evaluation of some factors. Dengue Bull 29:1–9
3.
Alvarez M, Rodriguez-Roche R, Bernardo L, Vazquez S, Morier L, Gonzalez D, Castro O, Kouri G, Halstead SB, Guzman MG (2006) Dengue hemorrhagic fever caused by sequential dengue 1–3 virus infections over a long time interval: Havana epidemic, 2001–2002. Am J Trop Med Hyg 75:1113–1117PubMed
4.
Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ (1991) Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci USA 88:7978–7982PubMedCrossRef
5.
Cabezas S, Rojas G, Pavon A, Alvarez M, Pupo M, Guillen G, Guzman MG (2008) Selection of phage-displayed human antibody fragments on dengue virus particles captured by a monoclonal antibody: application to the four serotypes. J Virol Methods 147:235–243PubMedCrossRef
6.
Calisher CH (2005) Persistent emergence of dengue. Emerg Infect Dis 11:738–739PubMed
7.
Cantelar de Francisco N, Fernandez A, Albert Molina L, Perez Balbis E (1981) Survey of dengue in Cuba. 1978–1979. Rev Cubana Med Trop 33:72–78PubMed
8.
de Oliveira Poersch C, Pavoni DP, Queiroz MH, de Borba L, Goldenberg S, dos Santos CN, Krieger MA (2005) Dengue virus infections: comparison of methods for diagnosing the acute disease. J Clin Virol 32:272–277PubMedCrossRef
9.
Desogus A, Burioni R, Ingianni A, Bugli F, Pompei R, Fadda G (2003) Production and characterization of a human recombinant monoclonal Fab fragment specific for influenza A viruses. Clin Diagn Lab Immunol 10:680–685PubMed
10.
Gubler DJ (1998) Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11:480–496PubMed
11.
Guzman MG, Kouri G (1996) Advances in dengue diagnosis. Clin Diagn Lab Immunol 3:621–627PubMed
12.
Guzman MG, Kouri G (2003) Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges. J Clin Virol 27:1–13PubMedCrossRef
13.
Guzman MG, Kouri G (2008) Dengue haemorrhagic fever integral hypothesis: confirming observations, 1987–2007. Trans R Soc Trop Med Hyg 102:522–523PubMedCrossRef
14.
Henchal EA, Gentry MK, McCown JM, Brandt WE (1982) Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 31:830–836PubMed
15.
Hermida C, Pupo M, Guzman MG, Gonzalez M, Marcet R (1992) Use of a dengue anti-complex monoclonal antibody in viral purification. Rev Cubana Med Trop 44:171–176
16.
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:52–53CrossRef
17.
Kouri G, Mas P, Guzman MG, Soler M, Goyenechea A, Morier L (1983) Dengue hemorrhagic fever in Cuba, 1981: rapid diagnosis of the etiologic agent. Bull Pan Am Health Organ 17:126–132PubMed
18.
Kouri G, Guzman MG, Valdes L, Carbonel I, del Rosario D, Vazquez S, Laferte J, Delgado J, Cabrera MV (1998) Reemergence of dengue in Cuba: a 1997 epidemic in Santiago de Cuba. Emerg Infect Dis 4:89–92PubMedCrossRef
19.
Kumarasamy V, Wahab AH, Chua SK, Hassan Z, Chem YK, Mohamad M, Chua KB (2007) Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods 140:75–79PubMedCrossRef
20.
Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV (1992) Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30:545–551PubMed
21.
Lorono Pino MA, Cropp CB, Farfan JA, Vorndam AV, Rodriguez Angulo EM, Rosado Paredes EP, Flores Flores LF, Beaty BJ, Gubler DJ (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes. Am J Trop Med Hyg 61:725–730PubMed
22.
Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffiths AD, Winter G (1991) By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J Mol Biol 222:581–597PubMedCrossRef
23.
McCafferty J, Griffiths AD, Winter G, DJ C (1990) Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348:552–554PubMedCrossRef
24.
Morens DM, Halstead SB, Repik PM, Putvatana R, Raybourne N (1985) Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. J Clin Microbiol 22:250–254PubMed
25.
PAHO (1994) Dengue and dengue hemorrhagic fever in the Americas: guidelines for prevention and control.Scientific publication no.548. PAHO, Washington
26.
Rodriguez-Roche R, Alvarez M, Guzman MG, Morier L, Kouri G (2000) Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells. J Clin Microbiol 38:3508–3510
27.
Rosario D, Alvarez M, Vazquez S, Amin N, Rodriguez-Roche R, Guzman MG (2001) Application of molecular method to the diagnosis and characterization of a dengue outbreak in Cuba. Biotecnologia Aplicada 18:203–206
28.
Salonen EM, Parren PW, Graus YF, Lundkvist A, Fisicaro P, Vapalahti O, Kallio-Kokko H, Vaheri A, Burton DR (1998) Human recombinant Puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics. J Gen Virol 79(Pt 4):659–665PubMed
29.
Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Yang HH, Lin TH, Huang JH (2002) Potential application of nonstructural protein NS1 serotype-specific immunoglobulin G enzyme-linked immunosorbent assay in the seroepidemiologic study of dengue virus infection: correlation of results with those of the plaque reduction neutralization test. J Clin Microbiol 40:1840–1844PubMedCrossRef
30.
Shu PY, Chen LK, Chang SF, Su CL, Chien LJ, Chin C, Lin TH, Huang JH (2004) Dengue virus serotyping based on envelope and membrane and nonstructural protein NS1 serotype-specific capture immunoglobulin M enzyme-linked immunosorbent assays. J Clin Microbiol 42:2489–2494PubMedCrossRef
31.
32.
Tan GH, Yusoff K, Seow HF, Tan WS (2007) A phage-displayed single chain variable fragment that interacts with hepatitis B core antigen: library construction, selection and diagnosis. J Clin Virol 38:49–56PubMedCrossRef
33.
Toth R, Harper K, Mayo M, Torrance L (1999) Fusion proteins of single-chain variable fragments derived from phage dispaly libraries are effective reagents for routine diagnosis of potato leafroll virus infection in potato. Phytopathology 89:1015–1021PubMedCrossRef
34.
Vazquez S, Valdes O, Pupo M, Delgado I, Alvarez M, Pelegrino JL, Guzman MG (2003) MAC-ELISA and ELISA inhibition methods for detection of antibodies after yellow fever vaccination. J Virol Methods 110:179–184PubMedCrossRef
35.
Vorndam AV, Kuno G (1997) Laboratory diagnosis of dengue virus infections. In: Gubler D, Kuno G (eds) Dengue and dengue hemorrhagic fever. CAB International, Wallingford, pp 313–334
36.
37.
Williamson RA, Burioni R, Sanna PP, Partridge LJ, Barbas CF, Burton DR (1993) Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries. Proc Natl Acad Sci USA 90:4141–4145PubMedCrossRef
38.
Xu H, Di B, Pan YX, Qiu LW, Wang YD, Hao W, He LJ, Yuen KY, Che XY (2006) Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: implications for early diagnosis and serotyping of dengue virus infections. J Clin Microbiol 44:2872–2878PubMedCrossRef
39.
Young PR, Hilditch PA, Bletchly C, Halloran W (2000) An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38:1053–1057PubMed
Metadata
Title
Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals
Authors
Sheila Cabezas
Gertrudis Rojas
Alequis Pavon
Lidice Bernardo
Yinet Castellanos
Mayling Alvarez
Maritza Pupo
Gerardo Guillen
Maria G. Guzman
Publication date
01-07-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-009-0401-1

Other articles of this Issue 7/2009

Archives of Virology 7/2009 Go to the issue

Brief Report

The complete genome sequences for a novel enterovirus type, enterovirus 96, reflect multiple recombinations

Brief Report

Bovine immunodeficiency virus: identification of a long terminal repeat sequence with enhanced promoter activity

Original Article

MDV-1 VP22: a transporter that can selectively deliver proteins into cells

Original Article

Influenza A virus matrix protein 1 interacts with hTFIIIC102-s, a short isoform of the polypeptide 3 subunit of human general transcription factor IIIC

Annotated Sequence Record

Complete genome sequence of a Tobacco necrosis virus D isolate from olive trees

Original Article

Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits