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01-10-2008 | Brief Report

Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus

Authors: Nobuhiko Nakashima, Yuri Nakamura

Published in: Archives of Virology | Issue 10/2008

Abstract

Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae. Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted.

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Title: Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus
Authors: Nobuhiko Nakashima
Yuri Nakamura
Publication date: 01-10-2008
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 10/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-008-0208-5

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