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Archives of Virology
Published in: Archives of Virology 7/2008

01-07-2008 | Brief Report

Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus

Authors: Carl Spetz, Roar Moe, Dag-Ragnar Blystad

Published in: Archives of Virology | Issue 7/2008

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Abstract

An infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus (PnMV) was generated. It consisted of 6,098 nucleotides and encoded a polyprotein of 219.5 kDa. Sequence comparisons indicated that this isolate shared 98.6% (nucleotide) and 97.1% (amino acid) identity with the previously sequenced isolate from Germany. RNA transcripts derived from this cDNA were infectious in Nicotiana benthamiana. However, plants did not present typical PnMV symptoms. Furthermore, RNA transcripts from this cDNA clone were not infectious in poinsettia. Serial propagation of this cDNA clone in N. benthamiana plants restored symptom induction in this host but did not re-establish infectivity in poinsettia.
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Metadata
Title
Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus
Authors
Carl Spetz
Roar Moe
Dag-Ragnar Blystad
Publication date
01-07-2008
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0109-7

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