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Surgery Today
Published in: Surgery Today 3/2018

01-03-2018 | Original Article

The features of adipose-derived stem cells in patients with inflammatory bowel diseases

Authors: Tsunekazu Mizushima, Tadafumi Fukata, Hiroshi Takeyama, Hidekazu Takahashi, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori

Published in: Surgery Today | Issue 3/2018

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Abstract

Purpose

The treatment outcomes for inflammatory bowel disease (IBD) have been improving, but the development of better therapies is needed. Stem cell therapy is promising, but little is known about the differences in adipose-derived stem cells (ADSCs) between IBD patients and healthy individuals.

Methods

ADSCs were isolated from subcutaneous adipose tissue (SAT) in IBD (Crohn’s disease, 3; ulcerative colitis, 2) and non-IBD (colorectal cancer, 5; breast cancer, 1) patients. We also analyzed the effects of tumor necrosis factor (TNF)-α on murine ADSCs.

Results

The numbers of stromal vascular fraction (SVF) cells per gram of SAT were 7.72 ± 3.03 × 105 in IBD and 8.51 ± 8.80 × 105 in non-IBD patients (p > 0.05). The proportions of ADSCs in SVF cells were 4.98 ± 2.61% in IBD and 1.02 ± 0.67% in non-IBD patients. The numbers of ADSCs per gram of SAT were 4.16 ± 2.96 × 104 in IBD and 0.88 ± 1.04 × 104 in non-IBD patients. The proportions and numbers of ADSCs were significantly higher in IBD patients than in non-IBD patients. TNF-α significantly facilitated the proliferation and motility of murine ADSCs.

Conclusion

These results showed the potential advantage of freshly isolated autologous ADSCs in IBD patients.
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Metadata
Title
The features of adipose-derived stem cells in patients with inflammatory bowel diseases
Authors
Tsunekazu Mizushima
Tadafumi Fukata
Hiroshi Takeyama
Hidekazu Takahashi
Naotsugu Haraguchi
Junichi Nishimura
Taishi Hata
Chu Matsuda
Hirofumi Yamamoto
Yuichiro Doki
Masaki Mori
Publication date
01-03-2018
Publisher
Springer Singapore
Published in
Surgery Today / Issue 3/2018
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI
https://doi.org/10.1007/s00595-017-1591-x

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