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Surgery Today
Published in: Surgery Today 1/2017

01-01-2017 | Original Article

Cell sheet technology for the regeneration of gastrointestinal tissue using a novel gastric perforation rat model

Authors: Shiro Tanaka, Kengo Kanetaka, Mio Fujii, Shinichiro Ito, Yusuke Sakai, Shinichiro Kobayashi, Kosho Yamanouchi, Fumihiko Fujita, Tamotsu Kuroki, Susumu Eguchi

Published in: Surgery Today | Issue 1/2017

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Abstract

Purpose

Anastomotic leakage is a major postoperative complication. While cell sheet technology has been gaining popularity in the clinical field, the utility of cell sheets for containing contaminated enteral perforation remains unclear. We established a new gastric perforation rat model and evaluated the efficacy of the myoblast cell sheet to prevent leakage.

Methods

To establish a suitable rat model, perforations of various sizes were made in the stomach. We then pasted the sheet onto the perforated stomach in our new model. After the operation, the rats were sacrificed and analyzed macroscopically and histologically. Serum levels of C-related protein and hyaluronic acid in the peritoneal cavity washing lavage were also evaluated.

Results

An incision 5 mm in length was found to be the most suitable for evaluation of the cell sheet performance. In the rats where the cell sheet had been pasted onto the perforation site, we noted less adhesion in the abdominal cavity and a significant reduction in the hyaluronic acid levels in the peritoneal cavity washing lavage.

Conclusions

We evaluated the efficacy of cell sheets for preventing leakage of enteral contents in a gastric perforation rat model. Myoblast cell sheets were able to markedly reduce the degree of both intra-abdominal adhesion and inflammation.
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Metadata
Title
Cell sheet technology for the regeneration of gastrointestinal tissue using a novel gastric perforation rat model
Authors
Shiro Tanaka
Kengo Kanetaka
Mio Fujii
Shinichiro Ito
Yusuke Sakai
Shinichiro Kobayashi
Kosho Yamanouchi
Fumihiko Fujita
Tamotsu Kuroki
Susumu Eguchi
Publication date
01-01-2017
Publisher
Springer Japan
Published in
Surgery Today / Issue 1/2017
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI
https://doi.org/10.1007/s00595-016-1360-2

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