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Acta Diabetologica
Published in: Acta Diabetologica 4/2013

01-08-2013 | Original Article

Retinal heat shock protein 25 in early experimental diabetes

Authors: Silvia Pinach, Davina Burt, Elena Berrone, Federica Barutta, Graziella Bruno, Massimo Porta, Paolo Cavallo Perin, Gabriella Gruden

Published in: Acta Diabetologica | Issue 4/2013

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Abstract

Diabetic retinopathy is the leading cause of blindness in adults, and oxidative stress has been pathogenically associated with retinal neurodegeneration. Cellular stresses induce expression of heat shock proteins (HSPs) and this results in cytoprotection. Our aim was to assess retinal expression of HSP25 in early experimental diabetes. Mice were rendered diabetic by streptozotocin injection. Ten weeks after diabetes onset retinal HSP25 expression were studied by real-time PCR, immunoblotting and immunohistochemistry (IHC). Expression of nitrotyrosine and Cu/Zn superoxide dismutase (SOD), was assessed by IHC and apoptosis by TUNEL. Retinal HSP25 mRNA and protein expression was significantly increased in diabetic as compared to non-diabetic animals and localised predominantly within the retinal ganglion cells (RGC) layer. This was paralleled overexpression of nitrotyrosine and SOD and enhanced apoptosis. In early experimental diabetes, HSP25 is overexpressed in the RGC layer in parallel with markers of oxidative stress and apoptosis.
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Metadata
Title
Retinal heat shock protein 25 in early experimental diabetes
Authors
Silvia Pinach
Davina Burt
Elena Berrone
Federica Barutta
Graziella Bruno
Massimo Porta
Paolo Cavallo Perin
Gabriella Gruden
Publication date
01-08-2013
Publisher
Springer Milan
Published in
Acta Diabetologica / Issue 4/2013
Print ISSN: 0940-5429
Electronic ISSN: 1432-5233
DOI
https://doi.org/10.1007/s00592-011-0346-1

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