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01-06-2019 | Original Article

Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

Authors: Iuri Moraes Neyrão, André Luiz Veiga Conrado, Verônica Takatsuka, Carlos Eduardo Malavasi Bruno, Venâncio Guedes de Azevedo

Published in: Comparative Clinical Pathology | Issue 3/2019

Abstract

The objective of this study was to evaluate and quantify the fat storage in the liver of lesser guitarfish Zapteryx brevirostris submitted to different periods of restriction and refeeding. In a water recirculation system, 21 animals were divided into three groups: control (n=7), fed every day; W1 (n=7), with one week of restriction and two of refeeding and W2 (n=7), with two weeks of restriction and two of refeeding. After 76 days, the animals were euthanized and liver samples were collected, paraffin embedded, stained with PAS and evaluated with stereological procedures and histopatological changes were recorded. The area occupied by fat in the liver of control group was higher than that found in groups W1 and W2 (p<0.001). Melano-macrophage center areas and diameters have risen with increased fasting period while there were swollen and necrotic hepatocytes. These results pointed to physiological adaptations and liver lesions caused by free radicals during fasting periods. Similarly to teleost fish, there was a consumption of lipid reserves while enhancing the gluconeogenesis during fasting.

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Title: Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles
Authors: Iuri Moraes Neyrão
André Luiz Veiga Conrado
Verônica Takatsuka
Carlos Eduardo Malavasi Bruno
Venâncio Guedes de Azevedo
Publication date: 01-06-2019
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 3/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02953-8

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Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

Abstract

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