Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Comparative Clinical Pathology
Published in: Comparative Clinical Pathology 3/2019

01-06-2019 | Growth Factors | Original Article

The beneficial effect of equine chorionic gonadotropin hormone (eCG) on the in vitro co-culture of bovine spermatogonial stem cell with Sertoli cells

Authors: Arezoo Kashfi, Reza Narenji Sani, Mahmood Ahmadi-hamedani

Published in: Comparative Clinical Pathology | Issue 3/2019

Login to get access

Abstract

Spermatogenesis is a complex developmental process that originates from spermatogonia stem cell. This process consists of sequential, highly organized steps of cell proliferation and differentiation resulting in the generation of functional spermatozoa. Many growth factors, hormones, and cell interactions of germ cells with Sertoli cells regulate these processes, and the failure of any of the processes can lead to male infertility The aim of the present study was to determine the effects of equine chorionic gonadotropin on spermatogonial stem cell colony formation and differentiation after in vitro co-culture with Sertoli cells. In this experimental study, Sertoli and spermatogonial stem cells were isolated from 3- to 5-month-old calves. Co-cultured Sertoli and spermatogonial stem cells were treated with equine chorionic gonadotropin in treatment groups before colony assay. The present study showed that equine chorionic gonadotropin increase colony number and decrease colony diameter. Equine chorionic gonadotropin can induce proliferative pathway and inhibit differentiation process in spermatogonia stem cell co-culture with Sertoli cells.
Literature
Alomar M, Zarkawi M, Alzoabi MA (2018) Analysis of Awassi sperm motility in two media at different evels of temperature, pH and osmolality. Iran J App Anim Sci 8:431–438
Anjamrooz SH, Movahedin M, Tiraihi T, Mowla SJ (2006) In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation. Reprod Fertil Dev 18:709–720CrossRefPubMed
Anway MD, Folmer J, Wright WW, Zirkin BR (2003) Isolation of sertoli cells from adult rat testes: an approach to ex vivo studies of sertoli cell function. Biol Reprod 68:996–1002CrossRefPubMed
Baarends W, Grootegoed J (1999) Molecular biology of male gametogenesis. Molecular biology in reproductive medicine New York, USA: parthenon publishing group 271-295
De Franca LR, Silva VA, Chiarini-Garcia H, Garcia SK, Debeljuk L (2000) Cell proliferation and hormonal changes during postnatal development of the testis in the pig. Biol Reprod 63:1629–1636CrossRefPubMed
De Franca LR, Avelar GF, Almeida FL (2005) Spermatogenesis and sperm transit through the epididymis in mammals with emphasis on pigs. Theriogenol 63:300–318CrossRef
Dym M, Cavicchia JC (1997) Further observations on the blood-testis barrier in monkeys. Biol Reprod 17:390–403CrossRef
Enders GC, May JJ II (1994) Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice. Dev Biol 163:331–340CrossRefPubMed
Izadyar F, Matthijs-Rijsenbilt JJ, Ouden KD, Creemers LB, Woelders H, de Rooij DG (2002) Development of a cryopreservation protocol for type a spermatogonia. J Androl 23:537–545PubMed
Kojima Y, Kominami K, Dohmae K, Nonomura N, Miki T, Okuyama A (1997) Cessation of spermatogenesis in juvenile spermatogonial depletion (jsd/jsd) mice. Int J Urol 4:500–507CrossRefPubMed
Kubota H, Avarbock MR, Brinster RL (2004) Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proc Natl Acad Sci U S A 101:16489–16494CrossRefPubMedPubMedCentral
Kumar TR (2005) What have we learned about gonadotropin function from gonadotropin subunit and receptor knockout mice? Reproduction 130:293–302CrossRefPubMed
Lamb DJ, Spotts GS, Shubhada S, Baker KR (1991) Partial characterization of a unique mitogenic activity secreted by rat Sertoli cells. Mol Cell Endocrinol 79:1–12CrossRefPubMed
McLean D, Russell LD, Griswold MD (2002) Biological activity and enrichment of spermatogonial stem cells in vitamin a-deficient and hyperthermia-exposed testes from mice based on colonization following germ cell transplantation. Biol Reprod 66:1374–1379CrossRefPubMed
Meehan T, Schlatt S, O'Bryan MK, de Kretser DM, Loveland KL (2000) Regulation of germ cell and Sertoli cell development by activin, follistatin, and FSH. Dev Biol 220:225–237CrossRefPubMed
Pierce JG, Parsons TF (1981) Glycoprotein hormones: structure and function. Annu Rev Biochem 50:465–495CrossRef
Tajik P, Sani RN, Moezifar M, Yousefi M, Movahedin M, Qasemi-Panahi B (2014) Effect of follicle-stimulating hormone and testosterone on colony formation of bovine spermatogonial stem cell. Comp Clin Pathol 23:901–906CrossRef
van Pelt AM, Morena AR, van Dissel-Emiliani FM, Boitani C, Gaemers IC, de Rooij DG (1996) Isolation of the synchronized A spermatogonia from adult vitamin A-deficient rat testes. Biol Reprod 55:439–444CrossRefPubMed
Yoshinaga K, Nishikawa S, Ogawa M, Hayashi S, Kunisada T, Fujimoto T (1991) Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development 113:689–699PubMed
Metadata
Title
The beneficial effect of equine chorionic gonadotropin hormone (eCG) on the in vitro co-culture of bovine spermatogonial stem cell with Sertoli cells
Authors
Arezoo Kashfi
Reza Narenji Sani
Mahmood Ahmadi-hamedani
Publication date
01-06-2019
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 3/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-019-02944-9

Other articles of this Issue 3/2019

Comparative Clinical Pathology 3/2019 Go to the issue

Original Article

Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

Review Article

Bracken fern toxicity and its associated clinicopathological effects in humans and animals: a review

Original Article

Health status of indigenous and exotic chickens sold in live bird markets

Original Article

Assessing the possible causes of hemolytic anemia associated with lumpy skin disease naturally infected buffaloes

Original Article

Metabolic status and ultrasound traits of reproductive tract of estrus dairy cows in early postpartum period

Original Article

Serum biochemical reference values of Kyrgyz donkeys (Equus asinus)