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01-11-2018 | Original Article

A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice

Authors: Mohammad Mahdi Zangeneh, Akram Zangeneh, Maryam Almasi, Reza Tahvilian, Fariba Hosseini, Rohallah Moradi

Published in: Comparative Clinical Pathology | Issue 6/2018

Abstract

Inula britannica L. has been used in medicine as a theraputical agent. The aim of the present study was to assess the hepatoprotective property of I. britannica aqueous extract against streptozotocin (STZ)-induced diabetes in mice. In this study, 60 mice were used. Diabetes was experimentally induced by injection of STZ in 50 mice. The levels of blood glucose were evaluated by glucometer strips. Mice with plasma glucose level more than 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into six groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 30 mg/kg glibenclamide orally. Groups 4, 5, and 6 were given 30, 90, and 270 mg/kg, respectively, of I. britannica for 20 days orally. At 20th day, the mice were dissected; blood and liver samples collected for hematological, biochemical, and histological parameter analysis. Hematologically, I. britannica and glibenclamide could reduce significantly (p ≤ 0.05) the raised levels of WBC, platelet, and increased RBC number as compared to the untreated group. Biochemically, I. britannica at all dose (especially IB270) and glibenclamide could significantly (p ≤ 0.05) reduce the raised levels of glucose, ALP, AST, ALT, cholesterol, LDL, and increased HDL, SOD, and CAT levels as compared to the untreated group. Histologically, different doses of I. britannica (especially IB270) similar to glibenclamide could significantly (p ≤ 0.05) decreased the volume of the liver structures as compared to the untreated group. It concluded that I. britannica could ameliorate hyperglycemia and improved hepatic changes following diabetes in STZ-induced diabetic mice. So, according to these features, it could be used as an antidiabetic supplement or drug.

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Title: A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice
Authors: Mohammad Mahdi Zangeneh
Akram Zangeneh
Maryam Almasi
Reza Tahvilian
Fariba Hosseini
Rohallah Moradi
Publication date: 01-11-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 6/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2789-z

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A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice

Abstract

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